Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum

Autor(es)

Brancini, Guilherme T. P. ^[1] ; Ferreira, Marcia E. S. ^[1] ; Rangel, Drauzio E. N. ^[2] ; Braga, Gilberto U. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Brasil, BR-08230030 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: G3-GENES, GENOMES, GENETICS; v. 9, n. 9, p. 2951-2961, SEP 2019.
• Citações Web of Science: 0

Resumo

Light is an important stimulus for fungi as it regulates many diverse and important biological processes. Metarhizium acridum is an entomopathogenic fungus currently used for the biological control of insect pests. The success of this approach is heavily dependent on tolerance to environmental stresses. It was previously reported that light exposure increases tolerance to ultraviolet radiation in M. acridum. There is no information in the literature about how light globally influences gene expression in this fungus. We employed a combination of mRNA-Sequencing and high-throughput proteomics to study how light regulates gene expression both transcriptionally and post-transcriptionally. Mycelium was exposed to light for 5 min and changes at the mRNA and protein levels were followed in time-course experiments for two and four hours, respectively. After light exposure, changes in mRNA abundance were observed for as much as 1128 genes or 11.3% of the genome. However, only 57 proteins changed in abundance and at least 347 significant changes at the mRNA level were not translated to the protein level. We observed that light downregulated subunits of the eukaryotic translation initiation factor 3, the eIF5A-activating enzyme deoxyhypusine hydroxylase, and ribosomal proteins. We hypothesize that light is perceived as a stress by the cell that responds to it by reducing translational activity. Overall, our results indicate that light acts both as a signal and a stressor to M. acridum and highlight the importance of measuring protein levels in order to fully understand light responses in fungi. (AU)

• Processo FAPESP: 15/24305-0 - Avaliação dos efeitos da luz azul e da luz vermelha na tolerância ao estresse e no proteoma do fungo entomopatogênico Metarhizium acridum
• Beneficiário: Guilherme Thomaz Pereira Brancini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/11386-5 - Estudo mecanístico da percepção e da resposta a luz visível no fungo entomopatogênico Metarhizium acridum
• Beneficiário: Gilberto Úbida Leite Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/50518-6 - Genes relacionados com estresse são induzidos pela luz visível durante o crescimento micelial, resultando em aumento da tolerância de conídios a condições de estresse
• Beneficiário: Drauzio Eduardo Naretto Rangel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/06374-1 - Luz visível durante o crescimento induz aumento de tolerância de conídios a diferentes condições de estresse em fungos
• Beneficiário: Drauzio Eduardo Naretto Rangel
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores