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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum

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Author(s):
Brancini, Guilherme T. P. [1] ; Ferreira, Marcia E. S. [1] ; Rangel, Drauzio E. N. [2] ; Braga, Gilberto U. L. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Brasil, BR-08230030 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: G3-GENES, GENOMES, GENETICS; v. 9, n. 9, p. 2951-2961, SEP 2019.
Web of Science Citations: 0
Abstract

Light is an important stimulus for fungi as it regulates many diverse and important biological processes. Metarhizium acridum is an entomopathogenic fungus currently used for the biological control of insect pests. The success of this approach is heavily dependent on tolerance to environmental stresses. It was previously reported that light exposure increases tolerance to ultraviolet radiation in M. acridum. There is no information in the literature about how light globally influences gene expression in this fungus. We employed a combination of mRNA-Sequencing and high-throughput proteomics to study how light regulates gene expression both transcriptionally and post-transcriptionally. Mycelium was exposed to light for 5 min and changes at the mRNA and protein levels were followed in time-course experiments for two and four hours, respectively. After light exposure, changes in mRNA abundance were observed for as much as 1128 genes or 11.3% of the genome. However, only 57 proteins changed in abundance and at least 347 significant changes at the mRNA level were not translated to the protein level. We observed that light downregulated subunits of the eukaryotic translation initiation factor 3, the eIF5A-activating enzyme deoxyhypusine hydroxylase, and ribosomal proteins. We hypothesize that light is perceived as a stress by the cell that responds to it by reducing translational activity. Overall, our results indicate that light acts both as a signal and a stressor to M. acridum and highlight the importance of measuring protein levels in order to fully understand light responses in fungi. (AU)

FAPESP's process: 15/24305-0 - Evaluating the effects of blue and red light on the stress tolerance and the proteome of the entomopathogenic fungus Metarhizium acridum
Grantee:Guilherme Thomaz Pereira Brancini
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Regular Research Grants
FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Research Grants - Young Investigators Grants