Visible light during growth enhances conidial tolerance to different stress conditions in fungi

• Grant number: 10/06374-1
• Support type: Research Grants - Young Investigators Grants
• Duration: July 01, 2012 - April 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Drauzio Eduardo Naretto Rangel
• Grantee: Drauzio Eduardo Naretto Rangel
• Home Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated grant(s): 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions, AP.R
• Associated scholarship(s): 13/25964-2 - Visible light during mycelial growth alters gene expression and improves stress tolerance in entomopathogenic fungi, BP.DR

Abstract

Visible light exposure during growth influences primary and secondary metabolism, growth, sexual and asexual development, and pigment formation in many fungal species. However, little is known about the phenotypic effects of light during mycelial growth on the tolerance of the developing fungal conidia to different stress conditions. In this study, conidia of several fungal species from two different phyla (Ascomycota and Zygomycota) will be produced on potato dextrose agar plus yeast extract (PDAY) medium under continuous, alternated exposure to visible light or dark. The effect of different light wavelengths, from blue to red, will also be studied. The tolerances to UV-B radiation, heat, oxidative stress, osmotic stress, and genotoxic agent 4NQO of the conidia produced under the different light exposure conditions will be evaluated and compared with the tolerances of conidia produced on minimal medium in the dark, which is known to produce conidia tolerant to a variety of environmental stresses. The cellular mechanism in which light changes the stress tolerance will be studied based on differential gene expression. The results in the fungal stress-tolerance phenotypes and in the gene expression could provide information about the effect of light during mycelial growth on the stress tolerance of the developing conidia of plant pathogens, fungal antagonists, insect pathogens, and saprophytic fungi. The main hypothesis is that light during growth improves conidial tolerance to one or several stress conditions in some fungi. (AU)

Scientific publications

(30)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RANGEL, DRAUZIO E. N.; PIEDRABUENA, AQUILES E.; ROITMAN, ISAAC; MESSIAS, CLAUDIO L. Laboratory and field studies for the control of Chagas disease vectors using the fungusMetarhizium anisopliae. Archives of Insect Biochemistry and Physiology, v. 105, n. 4, SI DEC 2020. Web of Science Citations: 0.

COSTA, TACYANA P. C.; RODRIGUES, ELIANE M.; DIAS, LUCIANA P.; PUPIN, BRENO; FERREIRA, PAULO C.; RANGEL, DRAUZIO E. N. Different wavelengths of visible light influence the conidial production and tolerance to ultra-violet radiation of the plant pathogens Colletotrichum acutatum and Fusarium fujikuroi. European Journal of Plant Pathology, v. 159, n. 1 NOV 2020. Web of Science Citations: 0.

ALDER-RANGEL, ALENE; IDNURM, ALEXANDER; BRAND, ALEXANDRA C.; BROWN, ALISTAIR J. P.; GORBUSHINA, ANNA; KELLIHER, CHRISTINA M.; CAMPOS, CLAUDIA B.; LEVIN, DAVID E.; BELL-PEDERSEN, DEBORAH; DADACHOVA, EKATERINA; BAUER, FLORIAN F.; GADD, GEOFFREY M.; BRAUS, GERHARD H.; BRAGA, GILBERTO U. L.; BRANCINI, GUILHERME T. P.; WALKER, GRAEME M.; DRUZHININA, IRINA; POCSI, ISTVAN; DIJKSTERHUIS, JAN; AGUIRRE, JESUS; HALLSWORTH, JOHN E.; SCHUMACHER, JULIA; WONG, KOON HO; SELBMANN, LAURA; CORROCHANO, LUIS M.; KUPIEC, MARTIN; MOMANY, MICHELLE; MOLIN, MIKAEL; REQUENA, NATALIA; YARDEN, ODED; CORDERO, RADAMES J. B.; FISCHER, REINHARD; PASCON, RENATA C.; MANCINELLI, ROCCO L.; EMRI, TAMAS; BASSO, THIAGO O.; RANGEL, DRAUZIO E. N. The Third International Symposium on Fungal Stress - ISFUS. FUNGAL BIOLOGY, v. 124, n. 5, p. 235-252, MAY 2020. Web of Science Citations: 2.

ARAUJO, CLAUDINEIA A. S.; FERREIRA, PAULO C.; PUPIN, BRENO; DIAS, LUCIANA P.; AVALOS, JAVIER; EDWARDS, JESSICA; HALLSWORTH, JOHN E.; RANGEL, DRAUZIO E. N. Osmotolerance as a determinant of microbial ecology: A study of phylogenetically diverse fungi. FUNGAL BIOLOGY, v. 124, n. 5, p. 273-288, MAY 2020. Web of Science Citations: 3.

DIAS, LUCIANA P.; PEDRINI, NICOLAS; BRAGA, GILBERTO U. L.; FERREIRA, PAULO C.; PUPIN, BRENO; ARAUJO, CLAUDINEIA A. S.; CORROCHANO, LUIS M.; RANGEL, DRAUZIO E. N. Outcome of blue, green, red, and white light on Metarhizium robertsii during mycelial growth on conidial stress tolerance and gene expression. FUNGAL BIOLOGY, v. 124, n. 5, p. 263-272, MAY 2020. Web of Science Citations: 4.

MEDINA, EDGAR Q. A.; OLIVEIRA, ARIEL S.; MEDINA, HUMBERTO R.; RANGEL, DRAUZIO E. N. Serendipity in the wrestle between Trichoderma and Metarhizium. FUNGAL BIOLOGY, v. 124, n. 5, p. 418-426, MAY 2020. Web of Science Citations: 2.

RANGEL, DRAUZIO E. N.; ALDER-RANGEL, ALENE. History of the International Symposium on Fungal Stress - ISFUS, a dream come true!. FUNGAL BIOLOGY, v. 124, n. 5, p. 525-535, MAY 2020. Web of Science Citations: 1.

BRANCINI, GUILHERME T. P.; FERREIRA, MARCIA E. S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum. G3-GENES, GENOMES, GENETICS, v. 9, n. 9, p. 2951-2961, SEP 2019. Web of Science Citations: 0.

RANGEL, DRAUZIO E. N.; ROBERTS, DONALD W. Possible source of the high UV-B and heat tolerance of Metarhizium acridum (isolate ARSEF 324). Journal of Invertebrate Pathology, v. 157, p. 32-35, SEP 2018. Web of Science Citations: 2.

ALDER-RANGEL, ALENE; BAILAO, ALEXANDRE M.; DA CUNHA, ANDERSON F.; SOARES, CELIA M. A.; WANG, CHENGSHU; BONATTO, DIEGO; DADACHOVA, EKATERINA; HAKALEHTO, ELIAS; ELEUTHERIO, ELIS C. A.; FERNANDES, EVERTON K. K.; GADD, GEOFFREY M.; BRAUS, GERHARD H.; BRAGA, GILBERTO U. L.; GOLDMAN, GUSTAVO H.; MALAVAZI, IRAN; HALLSWORTH, JOHN E.; TAKEMOTO, JON Y.; FULLER, KEVIN K.; SELBMANN, LAURA; CORROCHANO, LUIS M.; KRESS, MARCIA R. VON ZESKA; BERTOLINI, MARIA CELIA; SCHMOLL, MONIKA; PEDRINI, NICOLAS; LOERA, OCTAVIO; FINLAY, ROGER D.; PERALTA, ROSANE M.; RANGEL, DRAUZIO E. N. The second International Symposium on Fungal Stress: ISFUS. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 386-399, JUN 2018. Web of Science Citations: 5.

ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 621-628, JUN 2018. Web of Science Citations: 1.

BRANCINI, GUILHERME T. P.; TONANI, LUDMILLA; RANGEL, DRAUZIO E. N.; ROBERTS, DONALD W.; BRAGA, GILBERTO U. L. Species of the Metarhizium anisopliae complex with diverse ecological niches display different susceptibilities to antifungal agents. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 563-569, JUN 2018. Web of Science Citations: 3.

DIAS, LUCIANA P.; ARAUJO, CLAUDINEIA A. S.; PUPIN, BRENO; FERREIRA, PAULO C.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. The Xenon Test Chamber Q-SUN (R) for testing realistic tolerances of fungi exposed to simulated full spectrum solar radiation. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 592-601, JUN 2018. Web of Science Citations: 5.

FERREIRA, PAULO C.; PUPIN, BRENO; RANGEL, DRAUZIO E. N. Stress tolerance of soil fungal communities from native Atlantic forests, reforestations, and a sand mining degraded area. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 400-409, JUN 2018. Web of Science Citations: 4.

OLIVEIRA, ARIEL S.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. Metarhizium robertsii illuminated during mycelial growth produces conidia with increased germination speed and virulence. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 555-562, JUN 2018. Web of Science Citations: 7.

RANGEL, DRAUZIO E. N.; FINLAY, ROGER D.; HALLSWORTH, JOHN E.; DADACHOVA, EKATERINA; GADD, GEOFFREY MICHAEL. Fungal strategies for dealing with environment- and agriculture-induced stresses. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 602-612, JUN 2018. Web of Science Citations: 4.

BRANCINI, GUILHERME T. P.; BACHMANN, LUCIANO; FERREIRA, MARCIA ELIANA DA S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Exposing Metarhizium acridum mycelium to visible light up-regulates a photolyase gene and increases photoreactivating ability. Journal of Invertebrate Pathology, v. 152, p. 35-37, FEB 2018. Web of Science Citations: 2.

KEYSER, CHAD A.; FERNANDES, EVERTON K. K.; RANGEL, DRAUZIO E. N.; NELSON FOSTER, R.; JECH, LARRY E.; CHRIS REUTER, K.; BLACK, LONNIE R.; JARONSKI, STEFAN; FLAKE, II, DARL D.; EVANS, EDWARD W.; ROBERTS, DONALD W. Laboratory bioassays and field-cage trials of Metarhizium spp. isolates with field-collected Mormon crickets (Anabrus simplex). BIOCONTROL, v. 62, n. 2, p. 257-268, APR 2017. Web of Science Citations: 5.

LUO, ZHIBING; REN, HUI; MOUSA, JARROD J.; RANGEL, DRAUZIO E. N.; ZHANG, YONGJUN; BRUNER, STEVEN D.; KEYHANI, NEMAT O. The PacC transcription factor regulates secondary metabolite production and stress response, but has only minor effects on virulence in the insect pathogenic fungus Beauveria bassiana. ENVIRONMENTAL MICROBIOLOGY, v. 19, n. 2, p. 788-802, FEB 2017. Web of Science Citations: 12.

CRAY, JONATHAN A.; CONNOR, MAIREAD C.; STEVENSON, ANDREW; HOUGHTON, JONATHAN D. R.; RANGEL, DRAUZIO E. N.; COOKE, LOUISE R.; HALLSWORTH, JOHN E. Biocontrol agents promote growth of potato pathogens, depending on environmental conditions. MICROBIAL BIOTECHNOLOGY, v. 9, n. 3, p. 330-354, MAY 2016. Web of Science Citations: 22.

BRANCINI, GUILHERME T. P.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Exposure of Metarhizium acridum mycelium to light induces tolerance to UV-B radiation. FEMS Microbiology Letters, v. 363, n. 6 MAR 2016. Web of Science Citations: 6.

ALVES, FLAVIA DE LIMA; STEVENSON, ANDREW; BAXTER, ESTHER; GILLION, JENNY L. M.; HEJAZI, FAKHROSSADAT; HAYES, SANDRA; MORRISON, IAN E. G.; PRIOR, BERNARD A.; MCGENITY, TERRY J.; RANGEL, DRAUZIO E. N.; MAGAN, NARESH; TIMMIS, KENNETH N.; HALLSWORTH, JOHN E. Concomitant osmotic and chaotropicity-induced stresses in Aspergillus wentii: compatible solutes determine the biotic window. CURRENT GENETICS, v. 61, n. 3, p. 457-477, AUG 2015. Web of Science Citations: 44.

BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.; FERNANDES, EVERTON K. K.; FLINT, STEPHAN D.; ROBERTS, DONALD W. Molecular and physiological effects of environmental UV radiation on fungal conidia. CURRENT GENETICS, v. 61, n. 3, p. 405-425, AUG 2015. Web of Science Citations: 44.

FERNANDES, EVERTON K. K.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L.; ROBERTS, DONALD W. Tolerance of entomopathogenic fungi to ultraviolet radiation: a review on screening of strains and their formulation. CURRENT GENETICS, v. 61, n. 3, p. 427-440, AUG 2015. Web of Science Citations: 26.

RANGEL, DRAUZIO E. N.; ALDER-RANGEL, ALENE; DADACHOVA, EKATERINA; FINLAY, ROGER D.; DIJKSTERHUIS, JAN; BRAGA, GILBERTO U. L.; CORROCHANO, LUIS M.; HALLSWORTH, JOHN E. The International Symposium on Fungal Stress: ISFUS. CURRENT GENETICS, v. 61, n. 3, p. 479-487, AUG 2015. Web of Science Citations: 7.

RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L.; FERNANDES, EVERTON K. K.; KEYSER, CHAD A.; HALLSWORTH, JOHN E.; ROBERTS, DONALD W. Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation. CURRENT GENETICS, v. 61, n. 3, p. 383-404, AUG 2015. Web of Science Citations: 62.

STEVENSON, ANDREW; CRAY, JONATHAN A.; WILLIAMS, JIM P.; SANTOS, RICARDO; SAHAY, RICHA; NEUENKIRCHEN, NILS; MCCLURE, COLIN D.; GRANT, IRENE R.; HOUGHTON, JONATHAN D. R.; QUINN, JOHN P.; TIMSON, DAVID J.; PATIL, SATISH V.; SINGHAL, REKHA S.; ANTON, JOSEFA; DIJKSTERHUIS, JAN; HOCKING, AILSA D.; LIEVENS, BART; RANGEL, DRAUZIO E. N.; VOYTEK, MARY A.; GUNDE-CIMERMAN, NINA; OREN, AHARON; TIMMIS, KENNETH N.; MCGENITY, TERRY J.; HALLSWORTH, JOHN E. Is there a common water-activity limit for the three domains of life?. ISME Journal, v. 9, n. 6, p. 1333-1351, JUN 2015. Web of Science Citations: 76.

DE MENEZES, HENRIQUE D.; MASSOLA, JR., NELSON S.; FLINT, STEPHAN D.; SILVA, JR., GERALDO J.; BACHMANN, LUCIANO; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Growth under Visible Light Increases Conidia and Mucilage Production and Tolerance to UV-B Radiation in the Plant Pathogenic Fungus Colletotrichum acutatum. Photochemistry and Photobiology, v. 91, n. 2, p. 397-402, MAR-APR 2015. Web of Science Citations: 15.

AZEVEDO, ROSANA F. F.; SOUZA, ROBERTA K. F.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. Responsiveness of entomopathogenic fungi to menadione-induced oxidative stress. FUNGAL BIOLOGY, v. 118, n. 12, p. 990-995, DEC 2014. Web of Science Citations: 10.

SOUZA, ROBERTA K. F.; AZEVEDO, ROSANA F. F.; LOBO, ANDERSON O.; RANGEL, DRAUZIO E. N. Conidial water affinity is an important characteristic for thermotolerance in entomopathogenic fungi. BIOCONTROL SCIENCE AND TECHNOLOGY, v. 24, n. 4, p. 448-461, APR 3 2014. Web of Science Citations: 8.