|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||June 01, 2013|
|Effective date (End):||January 31, 2015|
|Field of knowledge:||Biological Sciences - Biochemistry - Molecular Biology|
|Principal Investigator:||Gilberto Úbida Leite Braga|
|Grantee:||Guilherme Thomaz Pereira Brancini|
|Home Institution:||Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil|
Conidia are specialized structures produced by many fungal species at a late developmental stage. Conidia are important for reproduction, dispersion and environmental persistence. For the entomopathogenic fungus Metarhizium spp, conidia are also responsible for host recognition and infection. Despite their importance, many aspects of conidial biology, including their proteomes, remain poorly known. Variations in physical (temperature, light) and/or chemical factors (substrate, pH) during fungal development may alter important characteristics of the conidia, for example, virulence and stress tolerance. It is known that light is a stimulus capable of increasing stress tolerance in Metarhizium spp. Our research group has already estabilished comparative proteome maps for the conidia and mycelium of M. acridum, employing two-dimensional electrophoresis (2-DE) and mass spectrometry (MALDI-TOF/TOF). These maps will be important to determine the effects of the environment on the conidial proteome. The main goals of this study is to compare the proteomes of conidia produced by colonies that grew in the presence to that grown in the absence of light and to identify proteins that may be related to stress tolerance against environmental factors such as ultraviolet radiation and temperature.