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Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum

Abstract

Visible light is an environmental factor that regulates metabolism, growth, reproduction and even pathogenicity and virulence in different species of fungi. Exposure to light can also pre-adapt fungal structures, such as conidium and mycelia, to future conditions of stress. In the entomopathogenic fungus Metarhizium acridum, exposure to visible light increases the tolerance to UV-B radiation (280-320 nm). As Metarhizium is used as mycoinsecticide the induction in tolerance to UV is highly desirable, since one of the factors that compromise its effectiveness is the inactivation of the inoculum by solar radiation. Despite the practical importance that the induction of UV tolerance has for the use of Metarhizium spp as mycoinsecticides, the molecular mechanisms that result in this effect have not been determined. The overall objective of the present project is to elucidate the molecular basis of the induction of UV tolerance by visible light in Metarhizium. The identification of the photoreceptors involved in the perception of light will be made by obtaining fungal strains delected to the genes for the major known photoreceptors (white collar-1, Cry-DASH, cryptochrome-2 and photolyase). Additionally, transcriptomic by deep sequencing of RNA (RNA-seq) and proteomic by LC-MS / MS approaches will be used in an integrated and complementary way, on the same samples, to identify genes and proteins involved in the response to visible light. It is expected that the results will provide information on the molecular mechanisms involved in the perception and response to light and also contribute to the production of M. acridum strains more tolerant to environmental stress, and therefore more suited to be used as micoinseticidas. (AU)

Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASTRO, KELLY A. D. F.; BRANCINI, GUILHERME T. P.; COSTA, LETICIA D.; BIAZZOTTO, JULIANA C.; FAUSTINO, M. AMPARO F.; TOME, AUGUSTO C.; NEVES, M. GRACA P. M. S.; ALMEIDA, ADELAIDE; HAMBLIN, MICHAEL R.; DA SILVA, ROBERTO S.; BRAGA, GILBERTO U. L. Efficient photodynamic inactivation ofCandida albicansby porphyrin and potassium iodide co-encapsulation in micelles. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 19, n. 8, p. 1063-1071, AUG 1 2020. Web of Science Citations: 2.
PEREIRA, LUIZ MIGUEL; MOTA, CAROLINE MARTINS; BARONI, LUCIANA; BRONZON DA COSTA, CASSIA MARIANA; VENANCIO BROCHI, JADE CABESTRE; WAINWRIGHT, MARK; PATRIARCA MINEO, TIAGO WILSON; LEITE BRAGA, GILBERTO UBIDA; YATSUDA, ANA PATRICIA. Inhibitory action of phenothiazinium dyes against Neospora caninum. SCIENTIFIC REPORTS, v. 10, n. 1 MAY 4 2020. Web of Science Citations: 0.
ALDER-RANGEL, ALENE; IDNURM, ALEXANDER; BRAND, ALEXANDRA C.; BROWN, ALISTAIR J. P.; GORBUSHINA, ANNA; KELLIHER, CHRISTINA M.; CAMPOS, CLAUDIA B.; LEVIN, DAVID E.; BELL-PEDERSEN, DEBORAH; DADACHOVA, EKATERINA; BAUER, FLORIAN F.; GADD, GEOFFREY M.; BRAUS, GERHARD H.; BRAGA, GILBERTO U. L.; BRANCINI, GUILHERME T. P.; WALKER, GRAEME M.; DRUZHININA, IRINA; POCSI, ISTVAN; DIJKSTERHUIS, JAN; AGUIRRE, JESUS; HALLSWORTH, JOHN E.; SCHUMACHER, JULIA; WONG, KOON HO; SELBMANN, LAURA; CORROCHANO, LUIS M.; KUPIEC, MARTIN; MOMANY, MICHELLE; MOLIN, MIKAEL; REQUENA, NATALIA; YARDEN, ODED; CORDERO, RADAMES J. B.; FISCHER, REINHARD; PASCON, RENATA C.; MANCINELLI, ROCCO L.; EMRI, TAMAS; BASSO, THIAGO O.; RANGEL, DRAUZIO E. N. The Third International Symposium on Fungal Stress - ISFUS. FUNGAL BIOLOGY, v. 124, n. 5, p. 235-252, MAY 2020. Web of Science Citations: 2.
DIAS, LUCIANA P.; PEDRINI, NICOLAS; BRAGA, GILBERTO U. L.; FERREIRA, PAULO C.; PUPIN, BRENO; ARAUJO, CLAUDINEIA A. S.; CORROCHANO, LUIS M.; RANGEL, DRAUZIO E. N. Outcome of blue, green, red, and white light on Metarhizium robertsii during mycelial growth on conidial stress tolerance and gene expression. FUNGAL BIOLOGY, v. 124, n. 5, p. 263-272, MAY 2020. Web of Science Citations: 4.
RODRIGUES, GABRIELA BRAGA; PINTO, MARCELO RODRIGUES; LEITE BRAGA, GILBERTO UBIDA. Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion. FUNGAL BIOLOGY, v. 124, n. 5, p. 297-303, MAY 2020. Web of Science Citations: 1.
BRANCINI, GUILHERME T. P.; FERREIRA, MARCIA E. S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum. G3-GENES, GENOMES, GENETICS, v. 9, n. 9, p. 2951-2961, SEP 2019. Web of Science Citations: 0.
ALVARENGA, VERONICA O.; BRANCINI, GUILHERME T. P.; SILVA, ERIC K.; DA PIA, ARTHUR K. R.; CAMPAGNOLLO, FERNANDA B.; BRAGA, GILBERTO U. L.; HUBINGER, MIRIAM D.; SANT'ANA, ANDERSON S. Survival variability of 12 strains of Bacillus cereus yielded to spray drying of whole milk. International Journal of Food Microbiology, v. 286, p. 80-89, DEC 2 2018. Web of Science Citations: 4.
ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 621-628, JUN 2018. Web of Science Citations: 1.
BRANCINI, GUILHERME T. P.; TONANI, LUDMILLA; RANGEL, DRAUZIO E. N.; ROBERTS, DONALD W.; BRAGA, GILBERTO U. L. Species of the Metarhizium anisopliae complex with diverse ecological niches display different susceptibilities to antifungal agents. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 563-569, JUN 2018. Web of Science Citations: 3.
OLIVEIRA, ARIEL S.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N. Metarhizium robertsii illuminated during mycelial growth produces conidia with increased germination speed and virulence. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 555-562, JUN 2018. Web of Science Citations: 7.
TONANI, LUDMILLA; MOROSINI, NATALIA SILVA; DE MENEZES, HENRIQUE DANTAS; NADALETTO BONIFACIO DA SILVA, MARIA EMILIA; WAINWRIGHT, MARK; LEITE BRAGA, GILBERTO UBIDA; VON ZESKA KRESS, MARCIA REGINA. In vitro susceptibilities of Neoscytalidium spp. sequence types to antifungal agents and antimicrobial photodynamic treatment with phenothiazinium photosensitizers. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 436-448, JUN 2018. Web of Science Citations: 4.
BRANCINI, GUILHERME T. P.; BACHMANN, LUCIANO; FERREIRA, MARCIA ELIANA DA S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L. Exposing Metarhizium acridum mycelium to visible light up-regulates a photolyase gene and increases photoreactivating ability. Journal of Invertebrate Pathology, v. 152, p. 35-37, FEB 2018. Web of Science Citations: 2.
GONZALES, JULIA C.; BRANCINI, GUILHERME T. P.; RODRIGUES, GABRIELA B.; SILVA-JUNIOR, GERALDO JOSE; BACHMANN, LUCIANO; WAINWRIGHT, MARK; BRAGA, GILBERTO U. L. Photodynamic inactivation of conidia of the fungus Colletotrichum abscissum on Citrus sinensis plants with methylene blue under solar radiation. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 176, p. 54-61, NOV 2017. Web of Science Citations: 6.

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