Mouse placental scaffolds: a three-dimensional environment model for recellularization

Autor(es)

Barreto, Rodrigo S. N. ^[1] ; Romagnolli, Patricia ^{[1, 2]} ; Fratini, Paula ^[1] ; Mess, Andrea Maria ^[1] ; Miglino, Maria Angelica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Butanta, SP - Brazil

^[2] Fed Univ Southern Frontier, Realeza, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TISSUE ENGINEERING; v. 10, AUG 2019.
• Citações Web of Science: 1

Resumo

The rich extracellular matrix (ECM) and availability make placenta eligible as alternative biomaterial source. Herein we produced placental mouse scaffolds by decellularization, and structure, composition, and cytocompatibility were evaluated to be considered as a biomaterial. We obtained a cell-free scaffold containing 9.42 +/- 5.2 ng dsDNA per mg of ECM, presenting well-preserved structure and composition. Proteoglycans were widespread throughout ECM without cell nuclei and cell remnants. Collagen I, weak in native placenta, clearly appears in the scaffold after recellularization, opposite distribution was observed for collagen III. Fibronectin was well-observed in placental scaffolds whereas laminin and collagen IV were strong expressed. Placental scaffolds recellularization potential was confirmed after mouse embryonic fibroblasts 3D dynamic culture, resulting in massive scaffold repopulation with cell-cell interactions, cell-matrix adhesion, and maintenance of natural morphology. Our small size scaffolds provide a useful tool for tissue engineering to produce grafts and organ fragments, as well as for cellular biology purposes for tridimensional culture substrate. (AU)

• Processo FAPESP: 14/50844-3 - Matriz extracelular na saúde e matriz placentária na regeneração de tecidos
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Temático