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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mouse placental scaffolds: a three-dimensional environment model for recellularization

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Author(s):
Barreto, Rodrigo S. N. [1] ; Romagnolli, Patricia [1, 2] ; Fratini, Paula [1] ; Mess, Andrea Maria [1] ; Miglino, Maria Angelica [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Butanta, SP - Brazil
[2] Fed Univ Southern Frontier, Realeza, PR - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF TISSUE ENGINEERING; v. 10, AUG 2019.
Web of Science Citations: 1
Abstract

The rich extracellular matrix (ECM) and availability make placenta eligible as alternative biomaterial source. Herein we produced placental mouse scaffolds by decellularization, and structure, composition, and cytocompatibility were evaluated to be considered as a biomaterial. We obtained a cell-free scaffold containing 9.42 +/- 5.2 ng dsDNA per mg of ECM, presenting well-preserved structure and composition. Proteoglycans were widespread throughout ECM without cell nuclei and cell remnants. Collagen I, weak in native placenta, clearly appears in the scaffold after recellularization, opposite distribution was observed for collagen III. Fibronectin was well-observed in placental scaffolds whereas laminin and collagen IV were strong expressed. Placental scaffolds recellularization potential was confirmed after mouse embryonic fibroblasts 3D dynamic culture, resulting in massive scaffold repopulation with cell-cell interactions, cell-matrix adhesion, and maintenance of natural morphology. Our small size scaffolds provide a useful tool for tissue engineering to produce grafts and organ fragments, as well as for cellular biology purposes for tridimensional culture substrate. (AU)