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Functionalized tissue factory: bioengineering based on ecm interactions with biopolymers and bioprinting

Grant number: 21/05445-7
Support Opportunities:Research Projects - Thematic Grants
Duration: July 01, 2023 - June 30, 2028
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Maria Angelica Miglino
Grantee:Maria Angelica Miglino
Host Institution: Universidade de Marília (UNIMAR). Marília , SP, Brazil
Associated researchers: Alexander Birbrair ; Alysson Renato Muotri ; Ana Claudia Oliveira Carreira Nishiyama ; Ana Lucia Abreu Silva ; Andrigo Barboza de Nardi ; Aparecida Maria Fontes ; Áureo Evangelista Santana ; Bruno Watanabe Minto ; Catia Helena de Almeida Lima Massari ; Charles Roy Long ; Christina Ramires Ferreira ; Concepta Margaret Mcmanus Pimentel ; Darcy Wagner ; Fabiana Fernandes Bressan ; Jean-Pierre Lavoie ; Joaquim Mansano Garcia ; Lawrence Charles Smith ; Lilian de Jesus Oliveira ; Luis Gustavo Gosuen Gonçalves Dias ; Luís Vicente Franco de Oliveira ; Marcelo Melo Soares ; Marcílio Félix ; Mari Cleide Sogayar ; Marina Ragagnin de Lima ; Niels Olsen Saraiva Câmara ; Paola Castro Moraes ; Patricia Castelucci ; Paulo Aléscio Canola ; Rodrigo da Silva Nunes Barreto ; Samara Silva de Souza ; Silviene Novikoff ; Tais Harumi de Castro Sasahara ; Uri Adrian Prync Flato

Abstract

Tissue Engineering employs tissue decellularization, recellularization and 3D bioprinting for production of functionalized biomaterials for transplants. Organs decellularization yields acellular biomaterials/scaffolds in which the composition and 3D structure of the extracelular matrix (ECM) are preserved. This biomaterial, when recellularized and directly applied in vivo, can provide local stimuli arise at the implantation site for stem and progenitor cells migration and proliferation, as well as cell differentiation and/or modulation of the innate imune response. 3D bioprinting may be employed to optimize the bioactivity of the decellularized scaffold both by structural customization and by association to other molecules and materials, thereby amplifying its potential applications. This Project is based on the hypothesis that decellularization, recellularization, 3D bioprinting and implantation of functionalized tissue may be utilized to better understand in vivo chemotaxis of endogenous cells towards biofunctionalized tissues to validate them for application in Veterinary Regenerative Medicine. To test this hypothesis, in vitro and in vivo models will be used. One of the wide variety of biomaterials to be employed is placental tissue, which shares with tumors several mechanisms associated with oncogenesis, such as: invasion, angiogenesis and modulation of the ECM. Therefore, the Objectives of this Project are: a) to optimize the protocol for decellularization of several tissues, aiming at the generation of scaffolds which may be associated to different cell types (recellularization) and biopolymers, yielding functionalized tissues to be utilized for organ and tissue repair and for transplant in animal models; b) to investigate the effects of placenta-derived biomaterials genesis, progression of tumor. A multidisciplinary team involving anatomists, cell biologists, surgeons and immunologists and access to state-of-the-art technologies and facilities warrant the viability of this proposal. The results obtained should contribute to better understanding not only of the cell and molecular interactions occurring at the tissue and tumor sites, but, also, allow designing novel therapeutic strategies for tissue repair and intervention in tumor growth and dissemination. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARRETO, RODRIGO DA SILVA NUNES; DA SILVA JUNIOR, LEANDRO NORBERTO DA SILVA; ALMEIDA, GUSTAVO HENRIQUE DONA RODRIGUES; HORVATH-PEREIRA, BIANCA DE OLIVEIRA; DA SILVA, THAMIRES SANTOS; GARCIA, JOAQUIM MANSANO; SMITH, LAWRENCE CHARLES; CARREIRA, ANA CLAUDIA OLIVEIRA; MIGLINO, MARIA ANGELICA. Placental scaffolds as a potential biological platform for embryonic stem cells differentiation into hepatic-like cells lineage: A pilot study. TISSUE & CELL, v. 84, p. 10-pg., . (21/05445-7)
DONA RODRIGUES ALMEIDA, GUSTAVO HENRIQUE; DA SILVA-JUNIOR, LEANDRO NORBERTO; GIBIN, MARIANA SVERSUT; DOS SANTOS, HENRIQUE; DE OLIVEIRA HORVATH-PEREIRA, BIANCA; MAZO PINHO, LETICIA BEATRIZ; BAESSO, MAURO LUCIANO; SATO, FRANCIELLE; HERNANDES, LUZMARINA; LONG, CHARLES R.; et al. Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture. CELLS, v. 12, n. 14, p. 20-pg., . (21/05445-7)

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