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Resistance and compliance of decellularized lung scaffolds along recellularization with stem cells

Grant number: 12/04052-2
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 10, 2012
Effective date (End): July 09, 2013
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Luís Vicente Franco de Oliveira
Grantee:Paula Naomi Nonaka
Supervisor: Ramon Farre Ventura
Host Institution: Universidade Nove de Julho (UNINOVE). Campus Memorial. São Paulo , SP, Brazil
Research place: Universitat de Barcelona (UB), Spain  
Associated to the scholarship:11/21709-2 - Resistance and compliance of decellularized lung scaffolds along recellularization with stem cells., BP.DR


The matrix of decellularized organs potentially keeps the three-dimensional architecture and biochemical composition, and the microvasculature of the original tissue. These properties make the decellularized lung, very promising for the bioartificial generation of a functional lung, since it recreates a better microenvironment in vivo. The existing scientific evidence supports the idea that the physical microenvironment contributes significantly to the modulation of the differentiation of mesenchymal stem cells, derived from embryonic and bone marrow. However, no scientific data showing that the fate of induced pluripotent stem cells could be specifically affected by physical stimuli are available. The mechanical behavior of resistance and compliance of lungs in the process of recellularization of scafold lungs with stem cells is still unknown. Thus, this study aims to investigate in an experimental animal model, the behavior of the mechanical properties of decellularized rat lungs by different techniques of decellularization and during the process of stem cell recellularization. After the process of lungs decellularization it will be done the DNA and components of the extracellular matrix (ECM) quantification. During the stem cell recellularization, the lungs will be subjected to a bioreactor where they will suffer the action of physical stimuli that mimic ventilation. The mechanical parameters will be collected by the method of end-inspiratory occlusion. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NONAKA, PAULA N.; URIARTE, JUAN J.; CAMPILLO, NOELIA; MELO, ESTHER; NAVAJAS, DANIEL; FARRE, RAMON; OLIVEIRA, LUIS V. F.. Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate. Respiratory Physiology & Neurobiology, v. 200, p. 1-5, . (12/04052-2)
NONAKA, PAULA N.; CAMPILLO, NOELIA; URIARTE, JUAN J.; GARRETA, ELENA; MELO, ESTHER; DE OLIVEIRA, LUIS V. F.; NAVAJAS, DANIEL; FARRE, RAMON. Effects of freezing/thawing on the mechanical properties of decellularized lungs. Journal of Biomedical Materials Research Part A, v. 102, n. 2, p. 413-419, . (12/04052-2)

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