Resistance and compliance of lungs in decellularization and recellularization process with stem cell.

• Grant number: 13/21765-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2014
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Luís Vicente Franco de Oliveira
• Grantee: Nadua Apostólico
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated scholarship(s): 15/01649-6 - Resistance and compliance of lungs in decellularization and recellularization process with stem cell, BE.EP.MS

Abstract

The matrix of decellularized organs potentially maintains the three-dimensional architecture and biochemical composition, as well as the original tissue microvasculature. These properties make the lung decellularized very promising for generating bioartificial lung function, since it provides better rebuild the microenvironment in vivo. The existing scientific evidence supporting the idea that the physical microenvironment contributes significantly to the modulation of the differentiation of mesenchymal stem cells, embryonic and bone marrow derived. However, no data are available to date, showing the fate of induced pluripotent stem cells could be specifically affected by physical stimuli. The mechanical behavior of resistance and compliance of decellularized lungs in the process of repopulation with stem cells is still unknown. Therefore, this study aims to investigate in an experimental animal model, the behavior of the mechanical properties of decellularized lungs of rats by different decellularization techniques and during the process of stem cell repopulation. The mechanical parameters will be collected through the occlusion method at the end of inspiration. For the realization of this project is a partnership between the Experimental Laboratory of Mechanics Cardiorespiratory Program Graduate Masters and PhD in Rehabilitation Sciences UNINOVE of the study group of Biophysics and Bioengineering Laboratory, Barcelona University - Spain under the responsibility of Prof. Daniel Navajas and Prof. Ramon Farre.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, LUIS VICENTE FRANCO; APOSTOLICO, NADUA; URIARTE, JUAN JOSE; DA PALMA, RENATA KELLY; PRATES, RENATO A.; DEANA, ALESSANDRO MELO; FERREIRA, LUIS RODOLFO; AFONSO, JOAO PEDRO RIBEIRO; DE PAULA VIEIRA, RODOLFO; DE OLIVEIRA JUNIOR, MANOEL CARNEIRO; et al. Photodynamic Therapy in the Extracellular Matrix of Mouse Lungs: Preliminary Results of an Alternative Tissue Sterilization Process. INTERNATIONAL JOURNAL OF PHOTOENERGY, v. 2021, MAY 29 2021. (15/01649-6, 13/21765-5)

OLIVEIRA, EZEQUIEL FERNANDES; NACIF, SERGIO R.; PEREIRA, NIXON ALVES; FONSECA, NINA TEIXEIRA; URBANO, JESSICA JULIOTI; PEREZ, EDUARDO ARAUJO; CAVALCANTE, VALERIA; OLIVEIRA, CLAUDIA SANTOS; INSALACO, GIUSEPPE; OLIVEIRA, ACARY SOUSA BULLE; et al. Sleep disorders in patients with myasthenia gravis: a systematic review. Journal of Physical Therapy Science, v. 27, n. 6, p. 2013-2018, JUN 2015. (14/07262-3, 13/21765-5)