| Texto completo | |
| Autor(es): |
Moura, Alexandre S.
[1]
;
Cardoso, Andre F.
[1]
;
Costa-da-Silva, Andre L.
[1, 2]
;
Winter, Carlos E.
[1]
;
Tania Bijovsky, A.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, SP - Brazil
[2] INCT EM, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 10, n. 2 FEB 24 2015. |
| Citações Web of Science: | 3 |
| Resumo | |
Despite the established role of Culex quinquefasciatus as a vector of various neurotropic viruses, such as the Rift Valley and West Nile viruses, as well as lymphatic filariasis, little is known regarding the organism's reproductive physiology. As in other oviparous animals, vitellogenin, the most important source of nutrients for the embryo development, is digested by intracellular proteases. Using mass spectrometry, we have identified two cathepsin B homologues partially purified by self-proteolysis of Cx. quinquefasciatus total egg extract. The transcriptional profile of these two cathepsin B homologues was determined by quantitative RT-PCR, and the enzymatic activity associated with the peptidase was determined in ovaries after female engorgement. According to the VectorBase (vectorbase.org) annotation, both cathepsin B homologues shared approximately 66% identity in their amino acid sequences. The two cathepsin B genes are expressed simultaneously in the fat body of the vitellogenic females, and enzymatic activity was detected within the ovaries, suggesting an extra-ovarian origin. Similar to the transcriptional profile of vitellogenin, cathepsin B transcripts were shown to accumulate post-blood meal and reached their highest expression at 36 h PBM. However, while vitellogenin expression decreased drastically at 48 h PBM, the expression of the cathepsins increased until 84 h PBM, at which time the females of our colony were ready for oviposition. The similarity between their transcriptional profiles strongly suggests a role for the cathepsin B homologues in vitellin degradation. (AU) | |
| Processo FAPESP: | 10/51241-0 - Culex quinquefasciatus: alguns aspectos da sua fisiologia |
| Beneficiário: | Alcira Tania Bijovsky de Katzin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/09211-4 - Culex quinquefasciatus: vitelogenina e suas proteases |
| Beneficiário: | Alcira Tania Bijovsky de Katzin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |