Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells

Autor(es)

Balera Brito, Victor Gustavo ^{[1, 2, 3]} ; Chaves-Neto, Antonio Hernandes ^{[1, 3, 4]} ; de Barrosa, Thamine Landim ^{[1, 2, 3]} ; Penha Oliveira, Sandra Helena ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] SBFis UNESP, Programa Multicentr Posgrad Ciencias Fisiol, Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Lab Pharmacol, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rodovia Marechal Candido Rondon SP-300, Km 527-528, BR-16018850 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Lab Biochem, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 244, NOV 15 2019.
• Citações Web of Science: 0

Resumo

Ethnopharmacological relevance: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. Aim of the study: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). Materials and methods: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 mu g/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, beta-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. Results: SYM (10 mu g/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-alpha and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. Conclusions: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro. (AU)

• Processo FAPESP: 11/06070-5 - Estudo do fenótipo osteoblástico em células-tronco mesenquimais de ratos normotensos e espontaneamente hipertensos (SHR) na presença ou não de doença periodontal
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/19458-1 - Avaliação dos efeitos da alta concentração de glicose e da participação da via insulínica sobre a diferenciação in vitro em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos espontaneamente hipertensos
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/20547-1 - Avaliação dos efeitos do extrato aquoso de erva-mate (Ilex paraguariensis) durante a diferenciação in vitro em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos Wistar
• Beneficiário: Victor Gustavo Balera Brito
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica