Assessing Genotoxicity and Mutagenicity of Three Common Amphibian Species Inhabiting Agroecosystem Environment

Autor(es)

Goncalves, Macks Wendhell ^{[1, 2]} ; Marins de Campos, Calebe Bertolino ^{[1, 2]} ; Godoy, Fernanda Ribeiro ^[2] ; Gambale, Priscilla Guedes ^[3] ; Nunes, Hugo Freire ^[1] ; Nomura, Fausto ^{[4, 5]} ; Bastos, Rogerio Pereira ^{[3, 4, 5]} ; da Cruz, Aparecido Divino ^[2] ; de Melo e Silva, Daniela ^{[4, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Goias, Inst Ciencias Biol 1, Dept Genet, Lab Mutagenese, Programa Posgrad Genet & Biol Mol, Campus 2, BR-74001970 Goiania, Go - Brazil

^[2] Pontificia Univ Catolica Goias, Dept Biol, Nucleo Pesquisas Replicon, Goiania, Go - Brazil

^[3] Univ Estadual Maringa, Programa Posgrad Ecol Ambientes Aquat Continentai, Maringa, Parana - Brazil

^[4] Univ Fed Goias, Programa Posgrad Biodiversidade Anim, Goiania, Go - Brazil

^[5] Univ Fed Goias, Inst Ciencias Biol, Programa Posgrad Ecol & Evolucao, Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Environmental Contamination and Toxicology; v. 77, n. 3, p. 409-420, OCT 2019.
• Citações Web of Science: 0

Resumo

Amphibians are constantly exposed to pollutants and the stress of agricultural activities. We selected three anuran amphibian species Dendropsophus minutus, Boana albopunctata, and Physalaemus cuvieri, totaling 309 individuals. We collected tadpoles in 15 permanent ponds: 5 soybean crops, 3 corn crops, and 7 nonagricultural lands. Our study provides the first comparative data on the genotoxicity and mutagenicity of three common amphibian anurans. Dendropsophus minutus was the most vulnerable species compared with B. albopunctata and P. cuvieri for comet assay and micronuclei test. However, the more significant amount of DNA damage seen in D. minutus does not mean that their populations are threatened once such species adapt well to anthropogenic disturbances. Despite, P. cuvieri was less sensitive than the other two species; the DNA damage was significantly higher in soybean crops. Physalaemus cuvieri is a leptodactylidae species that deposit their eggs in foam nests, which are essential to protect eggs from dehydration. Moreover, the foam reduces the contact of eggs with water; thus, P. cuvieri eggs could be less exposed to contaminants present in pounds, compared with D. minutus and B. albopunctata, which deposit their eggs directly in the water. Therefore, this study was sufficiently sensitive to detect genotoxic and mutagenic effects in tadpoles exposed to agroecosystems. We strongly suggest D. minutus in future biomonitoring studies that involve the comparison of anthropized versus not anthropized environments. Overall, we recommend the comet assay and micronucleus test as effective methods for the detection of genotoxic damage in amphibian anurans to the environmental disturbance, especially in agricultural sites. {[}GRAPHICS] . (AU)

• Processo FAPESP: 10/52321-7 - Girinos de anuros da Mata Atlântica, da Amazônia, do Pantanal, do cerrado e de zonas de transição: caracterização morfológica, distribuição espacial e padrões de diversidade
• Beneficiário: Denise de Cerqueira Rossa-Feres
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular