Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration

Autor(es)

Coelho, Fernanda ^[1] ; Cavicchioli, Mauricio ^[2] ; Specian, Sybele Saska ^[2] ; Scarel-Caminaga, Raquel Mantuaneli ^[1] ; Penteado, Leticia de Aquino ^[3] ; de Medeiros, Alexandra Ivo ^[3] ; de Lima Ribeiro, Sidney Jose ^[2] ; de Oliveira Capote, Ticiana Sidorenko ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Morphol, Sch Dent, Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Gen & Inorgan Chem, Inst Chem, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Biol Sci, Sch Pharmaceut Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 8 AUG 19 2019.
• Citações Web of Science: 2

Resumo

Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein. (AU)

• Processo FAPESP: 16/25926-1 - Desenvolvimento, avaliação da toxicidade e do potencial reparador ósseo de materiais à base de fibroína ou celulose bacteriana associados à hidroxiapatita e anticorpos anti-BMP-2.
• Beneficiário: Ticiana Sidorenko de Oliveira Capote
• Modalidade de apoio: Auxílio à Pesquisa - Regular