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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration

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Author(s):
Coelho, Fernanda [1] ; Cavicchioli, Mauricio [2] ; Specian, Sybele Saska [2] ; Scarel-Caminaga, Raquel Mantuaneli [1] ; Penteado, Leticia de Aquino [3] ; de Medeiros, Alexandra Ivo [3] ; de Lima Ribeiro, Sidney Jose [2] ; de Oliveira Capote, Ticiana Sidorenko [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Morphol, Sch Dent, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Gen & Inorgan Chem, Inst Chem, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Biol Sci, Sch Pharmaceut Sci, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 14, n. 8 AUG 19 2019.
Web of Science Citations: 2
Abstract

Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein. (AU)

FAPESP's process: 16/25926-1 - Development, evaluation of toxicity and bone repair potential of fibroin or bacterial cellulose-based materials associeted with hydroxyapatite and anti-BMP-2 antibodies
Grantee:Ticiana Sidorenko de Oliveira Capote
Support Opportunities: Regular Research Grants