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Development, evaluation of toxicity and bone repair potential of fibroin or bacterial cellulose-based materials associeted with hydroxyapatite and anti-BMP-2 antibodies


Tissue engineering related to bone tissue aims to provide repair/regeneration that presents similar physical and biological properties to the natural bone with adequate reestablishment of its functions. In this context, alloplastic materials based on biopolymers, such as silk fibroin (SF) and bacterial cellulose (BC), have provided the synthesis of excellent biomaterials for bone repair/regeneration. These biopolymers are biocompatible because they have interesting mechanical characteristics, have a slow rate of in vivo degradation, and have the ability to be chemically modified. In order to give these biopolymers a better bioactivity to potentiate the local effectiveness of bone repair/regeneration, the objective of the present study is to synthesize a matrix based on SF and another based on BC, each one being associated with hydroxyapatite and antibody Anti-bone morphogenetic protein (anti-BMP-2). The matrices with immobilized anti-BMP-2 are expected to be effective in capturing endogenous BMP-2, conferring a different therapeutic approach and potential use for bone tissue engineering. Therefore, these matrices will be characterized physico-chemically, and, for analysis of the potential of these matrices in bone repair/regeneration, in vitro assays will be performed to investigate cytotoxicity, genotoxicity, mutagenicity, gene expression and osteoblastic differentiation (Alkaline phosphatase) and detection of the formation of mineral nodules by Alizarin Red staining. Together, it is intended with this proposal to develop materials with a more efficient therapeutic approach for repair/ regeneration bone. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COELHO, FERNANDA; CAVICCHIOLI, MAURICIO; SPECIAN, SYBELE SASKA; CILLI, EDUARDO MAFFUD; LIMA RIBEIRO, SIDNEY JOSE; SCAREL-CAMINAGA, RAQUEL MANTUANELI; DE OLIVEIRA CAPOTE, TICIANA SIDORENKO. Silk fibroin/hydroxyapatite composite membranes: Production, characterization and toxicity evaluation. TOXICOLOGY IN VITRO, v. 62, FEB 2020. Web of Science Citations: 0.
COELHO, FERNANDA; CAVICCHIOLI, MAURICIO; SPECIAN, SYBELE SASKA; SCAREL-CAMINAGA, RAQUEL MANTUANELI; PENTEADO, LETICIA DE AQUINO; DE MEDEIROS, ALEXANDRA IVO; DE LIMA RIBEIRO, SIDNEY JOSE; DE OLIVEIRA CAPOTE, TICIANA SIDORENKO. Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration. PLoS One, v. 14, n. 8 AUG 19 2019. Web of Science Citations: 2.

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