Spatial heterogeneity and hydrological fluctuations drive bacterioplankton community composition in an Amazon floodplain system

Autor(es)

dos Reis, Mariana Camara ^{[1, 2, 3]} ; Bagatini, Inessa Lacativa ^[4] ; Vidal, Luciana de Oliveira ^[5] ; Bonnet, Marie-Paule ^{[6, 7]} ; Marques, David da Motta ^[8] ; Sarmento, Hugo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Programa Posgrad Ecol & Recursos Nat, Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Lab Microbial Proc & Biodivers, Dept Hidrobiol, Sao Carlos, SP - Brazil

^[3] Sorbonne Univ, CNRS, UMR 7144, Adaptat & Divers Milieu Marin, Stn Biol Roscoff, Pl Georges Teissier, Roscoff - France

^[4] Univ Fed Sao Carlos, Lab Ficol, Dept Bot, Sao Carlos, SP - Brazil

^[5] Univ Estadual Norte Fluminense, Lab Ciencias Ambientais, Ctr Biociencias & Biotecnol, Campos Dos Goytacazes, RJ - Brazil

^[6] Inst Res Dev, UMR Espace DEV 228, Montpellier - France

^[7] Univ Brasilia, Inst Res Dev, Int Joint Lab, LMI OCE, Brasilia, DF - Brazil

^[8] Univ Fed Rio Grande do Sul, Inst Hydraul Res, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 8 AUG 9 2019.
• Citações Web of Science: 0

Resumo

Amazonian floodplains form complex hydrological networks that play relevant roles in global biogeochemical cycles, and bacterial degradation of the organic matter in these systems is key for regional carbon budget. The Amazon undergoes extreme seasonal variations in water level, which produces changes in landscape and diversifies sources of organic inputs into floodplain systems. Although these changes should affect bacterioplankton community composition (BCC), little is known about which factors drive spatial and temporal patterns of bacterioplankton in these Amazonian floodplains. We used high-throughput sequencing (Illumina MiSeq) of the V3-V4 region of the 16S rRNA gene to investigate spatial and temporal patterns of BCC of two size fractions, and their correlation with environmental variables in an Amazon floodplain lake (Lago Grande do Curuai). We found a high degree of novelty in bacterioplankton, as more than half of operational taxonomic units (OTUs) could not be classified at genus level. Spatial habitat heterogeneity and the flood pulse were the main factors shaping free-living (FL) BCC. The gradient of organic matter from transition zone lake -Amazon River was the main driver for particle-attached (PA) BCC. The BCC reflected the complexity of the system, with more variation in space than in time, although both factors were important drivers of the BCC in this Amazon floodplain system. (AU)

• Processo FAPESP: 13/18083-0 - Família Selenastraceae (Chlorococcales): avaliação de potências marcadores moleculares para DNA barcoding e para filogenia dos gêneros Monoraphidium e Ankistrodesmus
• Beneficiário: Inessa Lacativa Bagatini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado