Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Autor(es)

Guilger-Casagrande, Mariana ^[1] ; Germano-Costa, Tais ^[1] ; Pasquoto-Stigliani, Tatiane ^[1] ; Fraceto, Leonardo Fernandes ^[2] ; de Lima, Renata ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sorocaba UNISO, Lab Evaluat Bioact & Toxicol Nanomat, Sorocaba, SP - Brazil

^[2] State Univ Sao Paulo UNESP, Lab Environm Nanotechnol, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, OCT 4 2019.
• Citações Web of Science: 0

Resumo

Biogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however Ag NP-TS was more effective against mycelia! growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests. (AU)

• Processo FAPESP: 16/13692-6 - Nanopartículas biogênicas de prata baseadas em Trichoderma harzianum: diferentes condições de síntese, avaliação da cito/genotoxicidade e potencial inibitório de Sclerotinia sclerotiorum
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/13328-5 - Desenvolvimento e avaliação de nanopartiulas metálicas biogênicas (prata, titânio e ferro) baseadas em Trichoderma harzianum para aplicação agrícola
• Beneficiário: Renata de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático