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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

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Author(s):
Guilger-Casagrande, Mariana [1] ; Germano-Costa, Tais [1] ; Pasquoto-Stigliani, Tatiane [1] ; Fraceto, Leonardo Fernandes [2] ; de Lima, Renata [1]
Total Authors: 5
Affiliation:
[1] Univ Sorocaba UNISO, Lab Evaluat Bioact & Toxicol Nanomat, Sorocaba, SP - Brazil
[2] State Univ Sao Paulo UNESP, Lab Environm Nanotechnol, Sorocaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, OCT 4 2019.
Web of Science Citations: 0
Abstract

Biogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however Ag NP-TS was more effective against mycelia! growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests. (AU)

FAPESP's process: 16/13692-6 - Silver biogenic nanoparticles based on Trichoderma harzianum: different synthesis conditions, evaluation of cyto/genotoxicity and inhibitory potential of Sclerotinia sclerotiorum
Grantee:Mariana Guilger Casagrande
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/13328-5 - Development and evaluation of biogenic metal nanoparticles (silver, titanium and iron) based on Trichoderma harzianum for agricultural application
Grantee:Renata de Lima
Support Opportunities: Regular Research Grants
FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
Grantee:Leonardo Fernandes Fraceto
Support Opportunities: Research Projects - Thematic Grants