Green synthesis and evaluation of iron nanoparticles based on Trichoderma harzianum for application in agriculture

• Grant number: 17/20932-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: April 01, 2018
• End date until: March 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Renata de Lima
• Grantee: Natalia Bilesky José
• Host Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil

Abstract

The production of nanomaterials applied to the agricultural area is a reality, this brings possibilities of application of the new technology as a promise for the solution of problems and consequent better crop yields. The use of metal nanoparticles for the control of pathogens is recent and one of the problems raised would be in regard to their toxicity both for the environment and for organisms. In order to reduce toxicity, there is a proposal for the use of biogenic nanoparticles, which present a capping composed of molecules from the reducing organism. The iron nanoparticles present low toxicity, which allows their application in the environment and the mycoparasite Trichoderma harzianum is an antagonist used in biological control, which in addition to pest control brings other benefits to crops, but this one faces the same sensitivity problems as others species used in pest control. Based on the above, the project presented here proposes to perform the synthesis of biogenic iron nanoparticles using the filtrate of Trichoderma harzianum as a reducing agent and stabilizer, with the purpose of application in the control of Sclerotinia sclerotiorum. The project aims to synthesize, characterize the nanoparticles, and evaluate their toxicity on different cell lines and microorganisms of agricultural importance and their effects on the soybean crop. The importance of this work is due to the need to develop solutions that are effective for the control of phytopathogens and reduce the environmental impacts, contributing to the guarantee of food and health. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUILGER-CASAGRANDE, MARIANA; GERMANO-COSTA, TAIS; BILESKY-JOSE, NATALIA; PASQUOTO-STIGLIANI, TATIANE; CARVALHO, LUCAS; FRACETO, LEONARDO F.; DE LIMA, RENATA. Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia scierotiorum. JOURNAL OF NANOBIOTECHNOLOGY, v. 19, n. 1, FEB 24 2021. (16/13692-6, 18/23608-8, 17/20932-6, 17/13328-5, 20/05816-2, 17/21004-5)

BILESKY-JOSE, NATALIA; MARUYAMA, CINTIA; GERMANO-COSTA, TAIS; CAMPOS, ESTEFANIA; CARVALHO, LUCAS; GRILLO, RENATO; FRACETO, LEONARDO FERNANDES; DE LIMA, RENATA. Biogenic alpha-Fe2O3 Nanoparticles Enhance the Biological Activity of Trichoderma against the Plant Pathogen Sclerotinia sclerotiorum. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 9, n. 4, p. 1669-1683, FEB 1 2021. (18/23608-8, 17/21004-5, 17/20932-6)

GUILGER-CASAGRANDE, MARIANA; BILESKY-JOSE, NATALIA; SOUSA, BRUNO TEIXEIRA; OLIVEIRA, HALLEY CAIXETA; FRACETO, LEONARDO FERNANDES; LIMA, RENATA. Effects of biogenic silver and iron nanoparticles on soybean seedlings (Glycine max). BMC PLANT BIOLOGY, v. 22, n. 1, p. 9-pg., 2022-05-24. (17/20932-6, 20/05816-2, 16/13692-6, 17/13328-5, 17/21004-5)

GERMANO-COSTA, T.; BILESKY-JOSE, N.; GUILGER-CASAGRANDE, M.; PASQUOTO-STIGLIANI, T.; ROGERIO, CB.; ABRANTES, DC.; MARUYAMA, CR.; OLIVEIRA, JL.; FRACETO, LF.; LIMA, R.. Use of 2D and co-culture cell models to assess the toxicity of zein nanoparticles loading insect repellents icaridin and geraniol. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 216, p. 12-pg., 2022-08-01. (20/05816-2, 17/20932-6, 18/14734-0, 15/18231-4, 17/25702-9, 18/02404-5)