B-1 cell-mediated modulation of M1 macrophage profile ameliorates microbicidal functions and disrupt the evasion mechanisms of Encephalitozoon cuniculi

Autor(es)

Pereira, Adriano ^{[1, 2]} ; Alvares-Saraiva, Anuska Marcelino ^{[3, 1, 4]} ; de Camargo Konno, Fabiana Toshie ^[1] ; Spadacci-Morena, Diva Denelle ^[3] ; Perez, Elizabeth Cristina ^[1] ; Mariano, Mario ^[1] ; Lallo, Maria Anete ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Paulista, Programa Patol Ambiental & Expt, Rua Jose Maria Whitaker, Sao Paulo, SP - Brazil

^[2] Ctr Univ Sao Camilo, Curso Biomed, Sao Paulo, SP - Brazil

^[3] Inst Butantan, Lab Fisiopatol, Sao Paulo, SP - Brazil

^[4] Univ Cruzeiro Sul, Programa Posgrad Interdisciplinar Ciencias Saude, Rua Galvao Bueno, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 13, n. 9 SEP 2019.
• Citações Web of Science: 0

Resumo

Author summary The adaptive immune response plays a key role against Encephalitozoon cuniculi, an opportunistic fungus for T cells immunodeficient patients. The role of B cells and antibody play in natural resistance to Encephalitozoon cuniculi remains unknown. Previously, we demonstrated that B-1 deficient mice (XID), an important component of innate immunity, were more susceptible to encephalitozoonosis, despite the increase in the number of CD4(+) and CD8(+) T lymphocytes. Here we observed that the absence of B-1 cells was associated with a larger population of M2 macrophages, a balance between anti-inflammatory and pro-inflammatory cytokines profile, which had lower microbicidal activity against E. cuniculi infection. However, in the presence of B-1 cells, peritoneal macrophages had a M1 profile with showed increased microbicidal activity and a higher percentage of apoptotic death. Here, we have investigated the possible effect of B-1 cells on the activity of peritoneal macrophages in E. cuniculi infection. In the presence of B-1 cells, peritoneal macrophages had an M1 profile with showed increased phagocytic capacity and index, associated with the intense microbicidal activity and a higher percentage of apoptotic death. The absence of B-1 cells was associated with a predominance of the M2 macrophages, reduced phagocytic capacity and index and microbicidal activity, increased pro-inflammatory and anti-inflammatory cytokines production, and higher percentual of necrosis death. In addition, in the M2 macrophages, spore of phagocytic E. cuniculi with polar tubular extrusion was observed, which is an important mechanism of evasion of the immune response. The results showed the importance of B-1 cells in the modulation of macrophage function against E. cuniculi infection, increasing microbicidal activity, and reducing the fungal mechanisms involved in the evasion of the immune response. (AU)

• Processo FAPESP: 15/25948-2 - Microsporidiose murina - o papel de macrófagos M1 e M2, fagócitos derivados de células B-1 e eferocitose
• Beneficiário: Maria Anete Lallo
• Modalidade de apoio: Auxílio à Pesquisa - Regular