Cytokine Profile in Early Infection by Leptospira interrogans in A/J Mice

Autor(es)

Bavia, Lorena ^[1] ; de Castro, Iris A. ^[1] ; Amano, Mariane Tami ^[1] ; Goncalves da Silva, Ana Maria ^[2] ; Vasconcellos, Silvio Arruda ^[3] ; Isaac, Lourdes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[3] Fac Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2019, OCT 7 2019.
• Citações Web of Science: 1

Resumo

Leptospirosis is considered a neglected disease with an estimated more than one million cases every year. Since rodents are at the same time the main reservoir and generally asymptomatic to Leptospira infection, understanding why some animal species are resistant and others are susceptible to this infection would shed some light in how to control this important zoonosis. The innate immune response against Leptospira is mainly dependent on phagocytosis and activation of the Complement System. In this context, cytokines may drive the early control of infection and the adaptive response. Since the Complement System is important to eliminate leptospires in vivo, we investigated if Complement C5 in A/J mice would modulate the cytokine production during infection by Leptospira interrogans serovar Kennewicki type Pomona Fromm (LPF). Thus, our aim was to investigate the systemic levels of pro- and anti-inflammatory cytokines during Leptospira infection in the blood, liver, lung, and kidney on the third and sixth days of infection in A/J C5(+/+) and A/J C5(-/-) mice. Blood levels of TNF-alpha, IL-6, IFN-gamma, and MCP-1 reached a peak on the third day. Although both mouse strains developed splenomegaly, similar histopathological alterations in the liver and the lung, levels of pro- and anti-inflammatory cytokines were different. A/J C5(+/+) mice had higher levels of liver IL-10, IL-1 beta, IL-12p40, and IL-12p70 and kidney IL-1 beta, IL-12p40, and IL-12p70 on the sixth day of infection when compared to A/J C5(-/-) mice. Our results showed that in A/J genetic background, the Complement component C5 modulates a cytokine profile in the liver and kidney of infected mice, which may play a role in the control of disease progression. (AU)

• Processo FAPESP: 11/15733-8 - Importância do componente C5 do sistema complemento para o controle de leptospirose in vivo em modelos murinos
• Beneficiário: Iris Arantes de Castro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/50043-0 - Sistema complemento e patogenicidade de leptospiras: mecanismos de ativação e escape identificação de ligantes bacterianos, caracterização de proteases e estabelecimento de modelo murino in vivo
• Beneficiário: Lourdes Isaac
• Modalidade de apoio: Auxílio à Pesquisa - Temático