Enzyme-assisted modification of flavonoids from Matricaria chamomilla: antioxidant activity and inhibitory effect on digestive enzymes

Autor(es)

Dini de Franco, Elida Paula ^[1] ; Contesini, Fabiano Jares ^[2] ; da Silva, Bianca Lima ^[1] ; Alves de Piloto Fernandes, Anna Maria ^[1] ; Leme, Camila Wielewski ^[3] ; Goncalves Cirino, Joao Pedro ^[1] ; Bueno Campos, Paula Renata ^[1] ; Carvalho, Patricia De Oliveira ^[1]

Afiliação do(s) autor(es)

^[1] Sao Francisco Univ USF, Lab Multidisciplinary Res, Braganca Paulista, SP - Brazil

^[2] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Enzyme Inhibition and Medicinal Chemistry; v. 35, n. 1, p. 42-49, JAN 1 2020.
• Citações Web of Science: 0

Resumo

Matricaria chamomilla L. contains antioxidant flavonoids that can have their bioactivity enhanced by enzymatic hydrolysis of specific glycosyl groups. This study implements an untargeted metabolomics approach based on ultra-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry technique operating in MSE mode (UPLC-QTOF-MSE) and spectrophotometric analysis of chamomile aqueous infusions, before and after hydrolysis by hesperidinase and ?-galactosidase. Several phenolic compounds were altered in the enzymatically treated infusion, with the majority being flavonoid derivatives of apigenin, esculetin, and quercetin. Although enzymatically modifying the infusion only led to a small increase in antioxidant activity (DPPH? method), its inhibitory effect on pancreatic lipase was of particular interest. The enzymatically treated infusion exhibited a greater inhibitory effect (EC50 of 35.6??M) than unmodified infusion and kinetic analysis suggested mixed inhibition of pancreatic lipase. These results are of great relevance due to the potential of enzymatically treated functional foods in human health. (AU)

• Processo FAPESP: 12/20393-4 - Avaliação em ratos do perfil lipidômico e biodisponibilidade de flavonóides naturais e biotransformados
• Beneficiário: Patrícia de Oliveira Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular