Cold Atmospheric Pressure Plasma Jet Reduces Trichophyton rubrum Adherence and Infection Capacity

Autor(es)

Borges, Aline Chiodi ^{[1, 2, 3]} ; Castaldelli Nishime, Thalita Mayumi ^{[4, 5]} ; Rovetta, Sabrina de Moura ^[1] ; Gouvea Lima, Gabriela de Morais ^[1] ; Kostov, Konstantin Georgiev ^[4] ; Thim, Gilmar Patrocinio ^[6] ; Canuto de Menezes, Beatriz Rossi ^[6] ; Barros Machado, Joao Paulo ^[7] ; Koga-Ito, Cristiane Yumi ^[1]

Afiliação do(s) autor(es)

^[1] Taubate Inst Higher Educ ITES, Dept Pharm, Ave Dom Pedro 1, 3575, BR-12090000 Taubate, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Environm Engn, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Oral Biopathol Grad Program, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Dept Chem & Phys, Guaratingueta Fac Engn, Av Dr Ariberto Pereira Cunha 333, BR-12516410 Guaratingueta, SP - Brazil

^[5] Leibniz Inst Plasma Sci & Technol INP, Felix Hausdorff St 2, D-17489 Greifswald - Germany

^[6] ITA, Div Fundamental Sci, Praca Marechal Eduardo Gomes 50, BR-12228970 Sao Jose Dos Campos, SP - Brazil

^[7] Natl Inst Space Res, Associated Lab Sensors & Mat, Ave Astronautas 1758, BR-12227010 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 184, n. 5, p. 585-595, OCT 2019.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of plasma jet on T. rubrum conidia germination and on mycelial growth were evaluated by in vitro assays. An ex vivo nail infection model was used to evaluate the effects on conidia adherence and infection. Biochemical analyses of nail fragments exposed or not to CAPP were performed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Plasma jet exposure for 10 and 15 min completely inhibited mycelial growth after only one exposure. Fifteen minutes of exposure could reduce conidia germination in suspension. Fungal suspensions exposed to plasma jet for 10 and 15 min were not able to infect nail specimens. These results were corroborated by ATR-FTIR analyses of nail fragments. In conclusion, single exposure to CAPP for 15 min was able to inhibit fungal growth, adherence and infection capacity. The results suggest that cold atmospheric plasma jet can be a promising alternative for the treatment of onychomycoses caused by T. rubrum. (AU)

• Processo FAPESP: 14/02354-7 - Atividade antifúngica e genotoxicidade do jato de plasma frio sob pressão atmosférica
• Beneficiário: Aline Chiodi Borges
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/21989-6 - Desenvolvimento de Jatos de Plasma Frio em Pressão Atmosférica para Tratamento de Materiais, Deposição de Filmes Finos e Aplicações Biomédicas
• Beneficiário: Konstantin Georgiev Kostov
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07196-6 - Prospecção de alternativas terapêuticas inovadoras para doenças infecciosas de interesse odontológico: jato de plasma frio sob pressão atmosférica e nanoencapsulado de substância bioativa
• Beneficiário: Cristiane Yumi Koga Ito
• Modalidade de apoio: Auxílio à Pesquisa - Regular