Cold Atmospheric Pressure Plasma Jet Reduces Trichophyton rubrum Adherence and Infection Capacity

Author(s)

Borges, Aline Chiodi ^{[1, 2, 3]} ; Castaldelli Nishime, Thalita Mayumi ^{[4, 5]} ; Rovetta, Sabrina de Moura ^[1] ; Gouvea Lima, Gabriela de Morais ^[1] ; Kostov, Konstantin Georgiev ^[4] ; Thim, Gilmar Patrocinio ^[6] ; Canuto de Menezes, Beatriz Rossi ^[6] ; Barros Machado, Joao Paulo ^[7] ; Koga-Ito, Cristiane Yumi ^[1]

Affiliation

^[1] Taubate Inst Higher Educ ITES, Dept Pharm, Ave Dom Pedro 1, 3575, BR-12090000 Taubate, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Environm Engn, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Oral Biopathol Grad Program, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Dept Chem & Phys, Guaratingueta Fac Engn, Av Dr Ariberto Pereira Cunha 333, BR-12516410 Guaratingueta, SP - Brazil

^[5] Leibniz Inst Plasma Sci & Technol INP, Felix Hausdorff St 2, D-17489 Greifswald - Germany

^[6] ITA, Div Fundamental Sci, Praca Marechal Eduardo Gomes 50, BR-12228970 Sao Jose Dos Campos, SP - Brazil

^[7] Natl Inst Space Res, Associated Lab Sensors & Mat, Ave Astronautas 1758, BR-12227010 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Mycopathologia; v. 184, n. 5, p. 585-595, OCT 2019.
• Web of Science Citations: 0

Abstract

This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of plasma jet on T. rubrum conidia germination and on mycelial growth were evaluated by in vitro assays. An ex vivo nail infection model was used to evaluate the effects on conidia adherence and infection. Biochemical analyses of nail fragments exposed or not to CAPP were performed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Plasma jet exposure for 10 and 15 min completely inhibited mycelial growth after only one exposure. Fifteen minutes of exposure could reduce conidia germination in suspension. Fungal suspensions exposed to plasma jet for 10 and 15 min were not able to infect nail specimens. These results were corroborated by ATR-FTIR analyses of nail fragments. In conclusion, single exposure to CAPP for 15 min was able to inhibit fungal growth, adherence and infection capacity. The results suggest that cold atmospheric plasma jet can be a promising alternative for the treatment of onychomycoses caused by T. rubrum. (AU)

• FAPESP's process: 14/02354-7 - Antifungal activity and genotoxicity of atmospheric pressure nonthermal plasma jet
• Grantee: Aline Chiodi Borges
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/21989-6 - Development of Cold Atmospheric Pressure Plasma Jets for Material Treatment, Thin Films Deposition and Biomedical Applications
• Grantee: Konstantin Georgiev Kostov
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance
• Grantee: Cristiane Yumi Koga Ito
• Support Opportunities: Regular Research Grants