Dry or wet? What is the best choice to determine gonadosomatic and hepatosomatic indexes in females of Macrobrachium amazonicum?

Autor(es)

Penido Paschoal, Lucas Rezende ^{[1, 2, 3]} ; Filgueira de Oliveira, Leo Jaime ^[4] ; Andrioli, Guilherme Casemiro ^[4] ; Zara, Fernando Jose ^{[4, 2, 5, 6]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Minas Gerais, Passos, MG - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, Biol Sci Zool, Rio Claro - Brazil

^[3] Fac Tecnol Nilo de Stefani FATEC, Jaboticabal - Brazil

^[4] Sao Paulo State Univ UNESP, Ctr Aquicultura CAUNESP, Jaboticabal - Brazil

^[5] Sao Paulo State Univ UNESP, UNESP CAUNESP, Sch Agr & Veterinarian Sci, Dept Biol, IML, Aquaculture Ctr, Jaboticabal - Brazil

^[6] Sao Paulo State Univ UNESP, IEAMar UNESP, Jaboticabal - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUACULTURE RESEARCH; v. 50, n. 12, p. 3589-3596, DEC 2019.
• Citações Web of Science: 0

Resumo

The gonadosomatic (GSI) and hepatosomatic (HSI) indexes are widely used for the evaluation of reproductive biology in crustaceans. However, the use of either wet or dry weight for estimating these indexes is not standardized in the literature. Here, we compare the GSI and HSI indexes based on wet and dry samples of females of Macrobrachium amazonicum from populations with different phenotypes (large and small-size prawns) as a model. In our analysis, for GSI, the application of wet weight tended to overestimate the values recorded in the initial (I-II) and intermediate (III) stages of ovarian development, and underestimate the results in the final (IV-V) stages when compared to the use of dry weight. This could be attributed to the reduction in cytoplasmic organelles and water content of the cytosol due to the increase in yolk and lipids in the oocytes throughout ovarian development. For HSI, no significant differences were detected between wet and dry samples. However, the dry HSI computed at all stages of development was overestimated when compared to the use of wet samples, and only the hepatopancreas of females at the final stage (V) showed a significant decrease in water content. Thus, our results indicate that M. amazonicum females maintain constant hepatopancreatic reserves throughout ovarian development. In this way, the use of dry weight is more adequate to estimate GSI and HSI in this species. (AU)

• Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
• Beneficiário: Fernando Luis Medina Mantelatto
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático