In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles

Autor(es):Mostrar menos -

Ducatti Formaggio, Daniela Maria ^[1] ; de Oliveira Neto, Xisto Antonio ^[2] ; Alcantara Rodrigues, Lina Dayse ^[1] ; de Andrade, Vitor Martins ^[2] ; Nunes, Bruna C. ^[1] ; Lopes-Ferreira, Monica ^[3] ; Ferreira, Fabiana G. ^[1] ; Wachesk, Cristiane C. ^[1] ; Camargo, Emerson R. ^[4] ; Conceicao, Katia ^[2] ; Tada, Dayane Batista ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Lab Nanomat & Nanotoxicol, Rua Talim 300, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Fed Sao Paulo, Lab Bioquim Peptideos, Rua Talim 300, Sao Jose Dos Campos, SP - Brazil

^[3] Inst Butantan, Special Lab Appl Toxinol CeTICS CEPID FAPESP, Immunoregulat Unit, Ave Vital Brasil 1500, Sao Paulo - Brazil

^[4] UFSCar Fed Univ Sao Carlos, Dept Chem, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOPARTICLE RESEARCH; v. 21, n. 11 NOV 2019.
• Citações Web of Science: 0

Resumo

Despite the potential antimicrobial activity of metallic nanoparticles, the increasing concerns about nanosafety have been holding back the use of these materials in therapeutics and biomedical devices. In the last years, several studies called attention to metallic nanoparticles toxicity. In the most part of in vitro studies performed with mammalian cells, metallic NPs reduced cell viability and induced genotoxicity and inflammatory responses. Bimetallic NPs have attracted great attention because they present distinct and even more advanced characteristics when compared to nanoparticles formed by a single metal. Recently, bimetallic NPs have emerged as an alternative to improve the antimicrobial activity of metallic nanoparticles, aiming at the broadening of the action spectrum and the reduction of the toxicity. However, the biocompatibility of bimetallic nanoparticles has been demonstrated only by in vitro studies. In the present work, the toxicity of AuPt nanoparticles was addressed both in vitro and in vivo. In addition, the antimicrobial activity of AuPt bimetallic nanoparticles has been evaluated in comparison with Au and Ag nanoparticles. The nanoparticles were characterized by ultraviolet-visible spectroscopy, dynamic light scattering, transmission electron microscopy, inductively coupled plasma optical emission spectroscopy, and X-ray diffraction. The antimicrobial activity was studied against Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The toxicity of nanoparticles was evaluated in vitro by analyzing their toxicity against human fibroblast cells (HS68 cell line) and in vivo by embryonic toxicity test in zebrafish (Danio rerio). The results confirmed the intrinsic antimicrobial activity of the three types of nanoparticles but different toxicity. Bimetallic nanoparticles showed enhanced antimicrobial activity in comparison with Au nanoparticles but lower antimicrobial activity compared with Ag nanoparticles. However, AuPt nanoparticles showed great advantage over Ag nanoparticles due to the absence of cytotoxicity and lower toxicity in vivo. (AU)

• Processo FAPESP: 17/01697-6 - Síntese e funcionalização de nanopartículas bimetálicas para aplicação na terapia fotodinâmica de câncer
• Beneficiário: Dayane Batista Tada
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23895-8 - Caracterização e controle de mecanismos de internalização celular de nanopartículas
• Beneficiário: Dayane Batista Tada
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores