Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice

Autor(es):Mostrar menos -

Portuondo, Deivys Leandro ^[1] ; Dores-Silva, Paulo Roberto ^[2] ; Ferreira, Lucas Souza ^[1] ; de Oliveira, Carlos S. ^[2] ; Tellez-Martinez, Damiana ^[1] ; Marcos, Caroline Maria ^[1] ; de Aguiar Loesch, Maria Luiza ^[1] ; Guzman, Fanny ^[3] ; Gava, Lisandra M. ^[4] ; Borges, Julio Cesar ^[2] ; Pereira, Sandro Antonio ^[5] ; Batista-Duharte, Alexander ^[1] ; Carlos, Iracilda Zeppone ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem, POB 780, BR-13560970 Sao Carlos, SP - Brazil

^[3] Pontificia Univ Catolica Valparaiso, NBC, Valparaiso - Chile

^[4] Univ Fed Sao Carlos, Ctr Biol & Hlth Sci, BR-13560970 Sao Carlos, SP - Brazil

^[5] Oswaldo Cruz Fdn Fiocruz, Evandro Chagas Natl Inst Infect Dis INI, Lab Clin Res Dermatozoonoses Domest Anim Lapclin, Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, NOV 20 2019.
• Citações Web of Science: 0

Resumo

In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-gamma and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis. (AU)

• Processo FAPESP: 17/13228-0 - Avaliação da modificação aminoacídica, caracterização estrutural e imunogenicidade dos peptídeos P1-A e P3-A derivados da enolase de S. schenckii para fins vacinais contra a esporotricose
• Beneficiário: Deivys Leandro Portuondo Fuentes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/26774-3 - Imunogenicidade e eficácia pré-clinica de um candidato vacinal de enolase recombinante contra Sporothrix schenckii e Sporothrix brasiliensis
• Beneficiário: Iracilda Zeppone Carlos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09340-4 - Identificação e síntese de peptídeos da enolase de Sporothrix schenckii como candidatos vacinais na esporotricose
• Beneficiário: Deivys Leandro Portuondo Fuentes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado