Colorimetric Paper-Based Immunosensor for Simultaneous Determination of Fetuin B and Clusterin toward Early Alzheimer's Diagnosis

Autor(es)

Brazaca, Lais C. ^{[1, 2]} ; Moreto, Jose R. ^[3] ; Martin, Aida ^[1] ; Tehrani, Farshad ^[1] ; Wang, Joseph ^[1] ; Zucolotto, Valtencir ^[2]

Afiliação do(s) autor(es)

^[1] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 - USA

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Nanomed & Nanotoxicol Grp, BR-13560970 Sao Carlos, SP - Brazil

^[3] San Diego State Univ, Dept Aerosp Engn, San Diego, CA 92182 - USA

• Tipo de documento: Artigo Científico
• Fonte: ACS NANO; v. 13, n. 11, p. 13325-13332, NOV 2019.
• Citações Web of Science: 0

Resumo

Alzheimer's disease is a devastating condition characterized by a progressive and slow brain decay in elders. Here, we developed a paper-based lateral flow immunoassay for simultaneous and fast determination of Alzheimer's blood biomarkers, fetuin B and clusterin. Selective antibodies to targeted biomarkers were immobilized on gold nanoparticles (AuNPs) and deposited on paper pads. After adding the sample on the paper-based device, the biofluid laterally flows toward the selective antibody, permitting AuNP-Ab accumulation on the test zone, which causes a color change from white to pink. Image analysis was performed using a customized algorithm for the automatic recognition of the area of analysis and color clustering. Colorimetric detection was compared to electrochemical methods for the precise quantification of biomarkers. The best performance was found for the color parameter ``L{*}{''}. Good linearity (R-2 = 0.988 and 0.998) and reproducibility (%RSD = 2.79% and 1.82%, N = 3) were demonstrated for the quantification of fetuin B and clusterin, respectively. Furthermore, the specificity of the immunosensor was tested on mixtures of proteins, showing negligible cross-reactivity and good performance in complex environments. We believe that our biosensor has a potential for early-stage diagnosis of Alzheimer's disease and toward a better understanding of Alzheimer's developing mechanisms. (AU)

• Processo FAPESP: 17/03779-0 - Aperfeiçoando biossensores baseados em papel para a quantificação de biomarcadores do Mal de Alzheimer usando técnicas de impressão
• Beneficiário: Laís Canniatti Brazaca
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/02623-0 - Novas Plataformas para o Diagnóstico Rápido e Precoce do Mal de Alzheimer
• Beneficiário: Laís Canniatti Brazaca
• Modalidade de apoio: Bolsas no Brasil - Doutorado