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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro and in vivo assessment of CaP materials for bone regenerative therapy. The role of multinucleated giant cells/osteoclasts in bone regeneration

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Autor(es):
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Cestari Bighetti, Ana Carolina [1] ; Cestari, Tania Mary [1] ; Santos, Paula Sanches [1] ; Nunes Arantes, Ricardo Vinicius [1] ; Paini, Suelen [1] ; Assis, Gerson Francisco [1] ; Costa, Bruna Carolina [2] ; de Oliveira, Flavia Amadeu [3] ; Tokuhara, Cintia Kazuko [3] ; de Oliveira, Rodrigo Cardoso [3] ; Taga, Rumio [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Bauru Sch Dent, Lab Histol, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[2] Sao Paulo State Univ, Phys Dept, Adv Mat Lab, UNESP, Ave Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Lab Biochem, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 108, n. 1, p. 282-297, JAN 2020.
Citações Web of Science: 2
Resumo

In this work, bone formation/remodeling/maturation was correlated with the presence of multinucleated giant cells (MGCs)/osteoclasts (tartrate-resistant acid phosphatase {[}TRAP]-positive cells) on the surface of beta-tricalcium phosphate (beta-TCP), sintered deproteinized bovine bone (sDBB), and carbonated deproteinized bovine bone (cDBB) using a maxillary sinus augmentation (MSA) in a New Zealand rabbit model. Microtomographic, histomorphometric, and immunolabeling for TRAP-cells analyses were made at 15, 30, and 60 days after surgery. In all treatments, a faster bone formation/remodeling/maturation and TRAP-positive cells activity occurred in the osteotomy region of the MSA than in the middle and submucosa regions. In the beta-TCP, the granules were rapidly reabsorbed by TRAP-positive cells and replaced by bone tissue. beta-TCP enabled quick bone regeneration/remodeling and full bone and marrow restoration until 60 days, but with a significant reduction in MSA volume. In cDBB and sDBB, the quantity of TRAP-positive cells was smaller than in beta-TCP, and these cells were associated with granule surface preparation for osteoblast-mediated bone formation. After 30 days, more than 80% of granule surfaces were surrounded and integrated by bone tissue without signs of degradation, preserving the MSA volume. Overall, the materials tested in a standardized preclinical model led to different bone formation/remodeling/maturation within the same repair process influenced by different microenvironments and MGCs/osteoclasts. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:282-297, 2020. (AU)

Processo FAPESP: 15/24898-1 - Avaliação microtomográfica e histomorfométrica da eficácia do xenoenxerto bovino (In700) e o aloplástico beta-tricálcio fosfato (B-TCP) em comparação ao Bio-Oss® no levantamento de seio maxilar de coelhos
Beneficiário:Ana Carolina Cestari Bighetti
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica