In vitro and in vivo assessment of CaP materials for bone regenerative therapy. The role of multinucleated giant cells/osteoclasts in bone regeneration

Author(s):Show less -

Cestari Bighetti, Ana Carolina ^[1] ; Cestari, Tania Mary ^[1] ; Santos, Paula Sanches ^[1] ; Nunes Arantes, Ricardo Vinicius ^[1] ; Paini, Suelen ^[1] ; Assis, Gerson Francisco ^[1] ; Costa, Bruna Carolina ^[2] ; de Oliveira, Flavia Amadeu ^[3] ; Tokuhara, Cintia Kazuko ^[3] ; de Oliveira, Rodrigo Cardoso ^[3] ; Taga, Rumio ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Lab Histol, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Sao Paulo State Univ, Phys Dept, Adv Mat Lab, UNESP, Ave Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Lab Biochem, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 108, n. 1, p. 282-297, JAN 2020.
• Web of Science Citations: 2

Abstract

In this work, bone formation/remodeling/maturation was correlated with the presence of multinucleated giant cells (MGCs)/osteoclasts (tartrate-resistant acid phosphatase {[}TRAP]-positive cells) on the surface of beta-tricalcium phosphate (beta-TCP), sintered deproteinized bovine bone (sDBB), and carbonated deproteinized bovine bone (cDBB) using a maxillary sinus augmentation (MSA) in a New Zealand rabbit model. Microtomographic, histomorphometric, and immunolabeling for TRAP-cells analyses were made at 15, 30, and 60 days after surgery. In all treatments, a faster bone formation/remodeling/maturation and TRAP-positive cells activity occurred in the osteotomy region of the MSA than in the middle and submucosa regions. In the beta-TCP, the granules were rapidly reabsorbed by TRAP-positive cells and replaced by bone tissue. beta-TCP enabled quick bone regeneration/remodeling and full bone and marrow restoration until 60 days, but with a significant reduction in MSA volume. In cDBB and sDBB, the quantity of TRAP-positive cells was smaller than in beta-TCP, and these cells were associated with granule surface preparation for osteoblast-mediated bone formation. After 30 days, more than 80% of granule surfaces were surrounded and integrated by bone tissue without signs of degradation, preserving the MSA volume. Overall, the materials tested in a standardized preclinical model led to different bone formation/remodeling/maturation within the same repair process influenced by different microenvironments and MGCs/osteoclasts. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:282-297, 2020. (AU)

• FAPESP's process: 15/24898-1 - Microtomographic and histomorphometric evaluation of the effectiveness of the bovine-derived xenograft (In700) and the alloplastic beta-tricalcium phosphate (B-TCP) compared to Bio-OssTM in sinus floor augmentation
• Grantee: Ana Carolina Cestari Bighetti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation