Landscape of heart proteome changes in a diet-induced obesity model

Autor(es):Mostrar menos -

Vileigas, Danielle F. ^[1] ; Harman, Victoria M. ^[2] ; Freire, Paula P. ^[3] ; Marciano, Cecilia L. C. ^[1] ; Sant'Ana, Paula G. ^[1] ; de Souza, Sergio L. B. ^[1] ; Mota, Gustavo A. F. ^[1] ; da Silva, Vitor L. ^[1] ; Campos, Dijon H. S. ^[1] ; Padovani, Carlos R. ^[4] ; Okoshi, Katashi ^[1] ; Beynon, Robert J. ^[2] ; Santos, Lucilene D. ^[5] ; Cicogna, Antonio C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618687 Botucatu, SP - Brazil

^[2] Univ Liverpool, Inst Integrat Biol, Ctr Proteome Res, Liverpool L69 7ZB, Merseyside - England

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, BR-18618970 Botucatu, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Grad Program Trop Dis FMB, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, DEC 2 2019.
• Citações Web of Science: 1

Resumo

Obesity is a pandemic associated with a high incidence of cardiovascular disease; however, the mechanisms are not fully elucidated. Proteomics may provide a more in-depth understanding of the pathophysiological mechanisms and contribute to the identification of potential therapeutic targets. Thus, our study evaluated myocardial protein expression in healthy and obese rats, employing two proteomic approaches. Male Wistar rats were established in two groups (n = 13/group): control diet and Western diet fed for 41 weeks. Obesity was determined by the adipose index, and cardiac function was evaluated in vivo by echocardiogram and in vitro by isolated papillary muscle analysis. Proteomics was based on two-dimensional gel electrophoresis (2-DE) along with mass spectrometry identification, and shotgun proteomics with label-free quantification. The Western diet was efficient in triggering obesity and impaired contractile function in vitro; however, no cardiac dysfunction was observed in vivo. The combination of two proteomic approaches was able to increase the cardiac proteomic map and to identify 82 differentially expressed proteins involved in different biological processes, mainly metabolism. Furthermore, the data also indicated a cardiac alteration in fatty acids transport, antioxidant defence, cytoskeleton, and proteasome complex, which have not previously been associated with obesity. Thus, we define a robust alteration in the myocardial proteome of diet-induced obese rats, even before functional impairment could be detected in vivo by echocardiogram. (AU)

• Processo FAPESP: 17/09688-6 - Proteoma miocárdico de ratos obesos por dieta ocidental com disfunção cardíaca: uma abordagem proteômica shotgun label-free
• Beneficiário: Danielle Fernandes Vileigas
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/16934-8 - Proteoma miocárdico de ratos obesos por dieta ocidental com disfunção cardíaca
• Beneficiário: Antonio Carlos Cicogna
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22152-0 - Proteoma miocárdico de ratos obesos por dieta ocidental com disfunção cardíaca
• Beneficiário: Danielle Fernandes Vileigas
• Modalidade de apoio: Bolsas no Brasil - Doutorado