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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Landscape of heart proteome changes in a diet-induced obesity model

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Author(s):
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Vileigas, Danielle F. [1] ; Harman, Victoria M. [2] ; Freire, Paula P. [3] ; Marciano, Cecilia L. C. [1] ; Sant'Ana, Paula G. [1] ; de Souza, Sergio L. B. [1] ; Mota, Gustavo A. F. [1] ; da Silva, Vitor L. [1] ; Campos, Dijon H. S. [1] ; Padovani, Carlos R. [4] ; Okoshi, Katashi [1] ; Beynon, Robert J. [2] ; Santos, Lucilene D. [5] ; Cicogna, Antonio C. [1]
Total Authors: 14
Affiliation:
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618687 Botucatu, SP - Brazil
[2] Univ Liverpool, Inst Integrat Biol, Ctr Proteome Res, Liverpool L69 7ZB, Merseyside - England
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, BR-18618970 Botucatu, SP - Brazil
[5] Sao Paulo State Univ UNESP, Grad Program Trop Dis FMB, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, DEC 2 2019.
Web of Science Citations: 1
Abstract

Obesity is a pandemic associated with a high incidence of cardiovascular disease; however, the mechanisms are not fully elucidated. Proteomics may provide a more in-depth understanding of the pathophysiological mechanisms and contribute to the identification of potential therapeutic targets. Thus, our study evaluated myocardial protein expression in healthy and obese rats, employing two proteomic approaches. Male Wistar rats were established in two groups (n = 13/group): control diet and Western diet fed for 41 weeks. Obesity was determined by the adipose index, and cardiac function was evaluated in vivo by echocardiogram and in vitro by isolated papillary muscle analysis. Proteomics was based on two-dimensional gel electrophoresis (2-DE) along with mass spectrometry identification, and shotgun proteomics with label-free quantification. The Western diet was efficient in triggering obesity and impaired contractile function in vitro; however, no cardiac dysfunction was observed in vivo. The combination of two proteomic approaches was able to increase the cardiac proteomic map and to identify 82 differentially expressed proteins involved in different biological processes, mainly metabolism. Furthermore, the data also indicated a cardiac alteration in fatty acids transport, antioxidant defence, cytoskeleton, and proteasome complex, which have not previously been associated with obesity. Thus, we define a robust alteration in the myocardial proteome of diet-induced obese rats, even before functional impairment could be detected in vivo by echocardiogram. (AU)

FAPESP's process: 17/09688-6 - MYOCARDIAL PROTEOME OF WESTERN DIET-INDUCED OBESE RATS WITH CARDIAC DYSFUNCTION: A LABEL-FREE SHOTGUN PROTEOMIC APPROACH
Grantee:Danielle Fernandes Vileigas
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 15/16934-8 - Myocardial proteome of obese rats by Western diet with cardiac dysfunction
Grantee:Antonio Carlos Cicogna
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22152-0 - Myocardial proteome of obese rats by Western diet with cardiac dysfunction
Grantee:Danielle Fernandes Vileigas
Support Opportunities: Scholarships in Brazil - Doctorate