In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Autor(es):Mostrar menos -

Pedroso, Reginaldo dos Santos ^{[1, 2, 3]} ; Balbino, Brenda Lorena ^[1] ; Andrade, Gessica ^[1] ; Pereira Sacardo Dias, Maria Cecilia ^[1] ; Alvarenga, Tavane Aparecida ^[1] ; Nascimento Pedroso, Rita Cassia ^[1] ; Pimenta, Leticia Pereira ^[1] ; Lucarini, Rodrigo ^[1] ; Pauletti, Patricia Mendonca ^[1] ; Januario, Ana Helena ^[1] ; Menezes Carvalho, Marco Tulio ^[4] ; Dantas Miranda, Mayker Lazaro ^[5] ; Pires, Regina Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

^[2] Univ Fed Uberlandia, Escola Tecn Saude, BR-38400902 Uberlandia, MG - Brazil

^[3] Univ Fed Uberlandia, Programa Posgrad Ciencias Saude, BR-38400902 Uberlandia, MG - Brazil

^[4] Univ Estadual Minas Gerais, BR-37902407 Passos, MG - Brazil

^[5] Inst Fed Triangulo Mineiro, Campus Uberlandia Ctr, BR-38064300 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANTS-BASEL; v. 8, n. 11 NOV 2019.
• Citações Web of Science: 0

Resumo

Candidiasis therapy, especially for candidiasis caused by Candida non-albicans species, is limited by the relatively reduced number of antifungal drugs and the emergence of antifungal tolerance. This study evaluates the anticandidal activity of 41 plant-derived products against Candida species, in both planktonic and biofilm cells. This study also evaluates the toxicity and the therapeutic action of the most active compounds by using the Caenorhabditis elegans-Candida model. The planktonic cells were cultured with various concentrations of the tested agents. The Cupressus sempervirens, Citrus limon, and Litsea cubeba essential oils as well as gallic acid were the most active anticandidal compounds. Candida cell re-growth after treatment with these agents for 48 h demonstrated that the L. cubeba essential oil and gallic acid displayed fungistatic activity, whereas the C. limon and C. sempervirens essential oils exhibited fungicidal activity. The C. sempervirens essential oil was not toxic and increased the survival of C. elegans worms infected with C. glabrata or C. orthopsilosis. All the plant-derived products assayed at 250 mu g/mL affected C. krusei biofilms. The tested plant-derived products proved to be potential therapeutic agents against Candida, especially Candida non-albicans species, and should be considered when developing new anticandidal agents. (AU)

• Processo FAPESP: 18/02333-0 - Estudo in vitro e in vivo dos efeitos de produtos vegetais frente a Candida no modo planctônico e biofilme de crescimento
• Beneficiário: Regina Helena Pires
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/20828-7 - Avaliação dos efeitos in vitro de derivados vegetais sobre células de leveduras
• Beneficiário: Maria Cecília Pereira Sacardo Dias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/26517-0 - Avaliação da atividade antifúngica de óleos essenciais frente a diferentes espécies de Candida
• Beneficiário: Brenda Lorena Balbino
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica