Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections

Autor(es)

Manrique, Wilson Gomez ^{[1, 2]} ; Pereira Figueiredo, Mayra Araguaia ^[2] ; Charlie-Silva, Ives ^[3] ; de Andrade Belo, Marco Antonio ^{[3, 1]} ; Dib, Cristina Corsi ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Jaboticabal Campus, Sao Paulo, SP - Brazil

^[2] Fed Univ Rondonia, Dept Vet Med, Rolim de Moura Campus, Rolim De Moura, Rondonia - Brazil

^[3] Brasil Univ, Sao Paulo, SP - Brazil

^[4] Biol Inst Sao Paulo, TB Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FISH & SHELLFISH IMMUNOLOGY; v. 95, p. 514-518, DEC 2019.
• Citações Web of Science: 0

Resumo

In order to understand the pathophysiology of melanomcrophage centers (MMCs) formation during the tilapia defense response to bacterial infections, the present study evaluated the response, in terms of area, number and pigment constitution, of splenic MMCs of Oreochromis niloticus subjected to intraperitoneal (i.p.) infection with Aeromonas hydrophila and Mycobacterium marinum. Eighty-four fish (396.9 +/- 21.0 g) were randomly distributed into twelve plastic tanks (300 L), to constitute three treatments with 28 animals each: control group (inoculated with PBS); Infected with A. hydrophila (1 x 10(7) UFC mL(-1)); Infected with M. marinum (1 x 10(6) UFC mL(-1)). The spleen was collected in seven fish per treatment on the 3rd, 7th, 14th and 21st day post-infection (DPI). The results revealed the participation of MMCs in the defense response of tilapia during bacterial infection by A. hydrophila and M. marinum, since there was an increase in the number and size of these cell aggregates. Variation of pigment accumulation with significant increase of hemosiderin, in infected tilapias by A. hydrophila, bacteria responsible for causing hemolytic anemia in fish was also found. On the other hand, M. marinum-infected tilapia had high amount of melanin in MMCs. In general, mycobacterial infections are notoriously difficult to treat, being characterized as a chronic disease. These findings demonstrate different strategies of fish response during the evolution of these bacterial diseases. (AU)

• Processo FAPESP: 13/01838-8 - Morfobiometria, constituição pigmentar, cito-imunoistoquímica de melanomacrófagos e centros de melanomacrófagos de Oreochromis niloticus inoculados com Aeromonas hydrophila e Mycobacterium marinum e expressão de receptores de membrana de fagócitos in vitr
• Beneficiário: Wilson Gómez Manrique
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado