Cellulose cone tip as a sorbent material for multiphase electrical field-assisted extraction of cocaine from saliva and determination by LC-MS/MS

Autor(es)

Sousa, Denise V. M. ^[1] ; Pereira, Fabiano V. ^[1] ; Nascentes, Clesia C. ^[1] ; Moreira, Juliane S. ^[1] ; Boratto, Victor H. M. ^[1] ; Orlando, Ricardo M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Dept Chem, Lab Microfluid & Separat LaMS, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 208, FEB 1 2020.
• Citações Web of Science: 0

Resumo

A porous and hydrophilic sorbent material was used in an extraction system, assisted by electric fields, for the extraction of cocaine in saliva and subsequent determination by ultra-high-performance liquid chromatography associated with sequential triple quadrupole mass spectrometry (UHPLC-MS/MS). The cellulose-based material was characterized by scanning electron microscopy, infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction. The time and voltage variables applied in the extraction process were investigated through a Doehlert experimental design, and with the best conditions found (35min and 300 V) some validation parameters were evaluated. The established working range was 1-100 mu g L-1 (R-2 > 0.99), and the detection and quantification limits determined were 0.3 and 0.8 mu g L-1, respectively. Recoveries from 80 to 115% and coefficient of variation <= 15 and 16% for intra-day and inter-day assays, respectively, were obtained for sample concentrations of LOQ, 5, 25, and 75 mu g L-1, indicating satisfactory accuracy and precision for the proposed method. In addition, the method presented no matrix effect, and the extraction efficiency was between 56 and 70%. The results showed that the material used has adequate physicochemical characteristics and can be applied as a sorbent and electrolyte support in multiphase extractions using electric fields. (AU)

• Processo FAPESP: 14/50951-4 - INCT 2014: Tecnologias Analíticas Avançadas
• Beneficiário: Celio Pasquini
• Modalidade de apoio: Auxílio à Pesquisa - Temático