| Full text | |
| Author(s): |
Sousa, Denise V. M.
[1]
;
Pereira, Fabiano V.
[1]
;
Nascentes, Clesia C.
[1]
;
Moreira, Juliane S.
[1]
;
Boratto, Victor H. M.
[1]
;
Orlando, Ricardo M.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Minas Gerais, Dept Chem, Lab Microfluid & Separat LaMS, BR-31270901 Belo Horizonte, MG - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Talanta; v. 208, FEB 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
A porous and hydrophilic sorbent material was used in an extraction system, assisted by electric fields, for the extraction of cocaine in saliva and subsequent determination by ultra-high-performance liquid chromatography associated with sequential triple quadrupole mass spectrometry (UHPLC-MS/MS). The cellulose-based material was characterized by scanning electron microscopy, infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction. The time and voltage variables applied in the extraction process were investigated through a Doehlert experimental design, and with the best conditions found (35min and 300 V) some validation parameters were evaluated. The established working range was 1-100 mu g L-1 (R-2 > 0.99), and the detection and quantification limits determined were 0.3 and 0.8 mu g L-1, respectively. Recoveries from 80 to 115% and coefficient of variation <= 15 and 16% for intra-day and inter-day assays, respectively, were obtained for sample concentrations of LOQ, 5, 25, and 75 mu g L-1, indicating satisfactory accuracy and precision for the proposed method. In addition, the method presented no matrix effect, and the extraction efficiency was between 56 and 70%. The results showed that the material used has adequate physicochemical characteristics and can be applied as a sorbent and electrolyte support in multiphase extractions using electric fields. (AU) | |
| FAPESP's process: | 14/50951-4 - Inct 2014 - tecnologias analiticas avancadas. |
| Grantee: | Celio Pasquini |
| Support Opportunities: | Research Projects - Thematic Grants |