Development of a monolithic phase with amino groups and nanoparticles for the determination of carbidopa, levodopa, benserazide, dopamine and 3-O-methyldopa in plasma samples from patients with Parkinson's disease by HILIC-MS / MS in column switching mode

• Grant number: 17/03726-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2017
• End date: May 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Eugênia Queiroz Nassur
• Grantee: Caroline Fernandes Grecco
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Parkinson's disease (PD) is an idiopathic neurodegenerative disease that affects about 3% of the Brazilian population over 65 years old. It can also be seen in individuals under 40 years old (Parkinsonism). PD is caused by deficiency of the neurotransmitter, dopamine, in the nigrostriatal and cortical pathway, mainly interfering in the motor system. Levodopa is an efficient prodrug (decarboxylated by the aromatic amino acid decarboxylase in dopamine) for the control of PD symptoms. Levodopa (LD) has been associated with peripheral dopa decarboxylase inhibitors, such as carbidopa or benserazide, to increase the availability of LD in the central nervous system and to reduce the adverse effects of dopamine formed in the peripheral system. Prolonged treatment of PD with LD can lead to fluctuations in motor performance and dyskinesias, that is, a decrease in the efficacy of LD. Liquid chromatography coupled to tandem mass spectrometry (LC-MS / MS) has been considered the benchmark analytical technique for the determination of drugs in biological fluids. Hydrophilic interaction liquid chromatography (HILIC) is an alternative mode of LC-MS / MS in the reserve phase for the determination of polar substances. The sample preparation step has been required in the development of LC-MS / MS methods, to eliminate the interferents and pre-concentrate the drugs, almost always present at trace levels in biological samples. Column switching liquidchromatography allows the automation of the analysis by hyphenation of the preparation stage of biological samples (pre-concentration of the analytes and removal of most of the endogenous components) with the analysis in the chromatographic system. In this project, the method employing hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry (HILIC-MS / MS) in column switching mode with monolithic silica capillary in the first dimension was standardized for the determination of carbidopa, levodopa, dopamine, 3-O-methyldopa and benserazide in plasma samples from patients with Parkinson's disease. After validation of the method, plasma concentrations of levodopa, 3-O-methyl-dopa, peripheral dopamine decarboxylase (carbidopa and benserazide) and dopamine inhibitor drugs will be determined in patients with Parkinson's disease on prolonged treatment with levodopa / carbidopa or levodopa / benserazide with symptoms of motor fluctuations (wearing-off) and dyskinesias. This study will aid in adjusting the doses of these patients.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GRECCO, CAROLINE FERNANDES; SOUZA, ISRAEL D.; COSTA QUEIROZ, MARIA EUGENIA. Recent development of chromatographic methods to determine parabens in breast milk samples: A review. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1093, p. 82-90, SEP 1 2018. (17/03726-3, 17/02147-0)

GRECCO, CAROLINE FERNANDES; MIRANDA, LUIS FELIPPE CABRAL; QUEIROZ, MARIA EUGENIA COSTA. Aminopropyl hybrid silica monolithic capillary containing mesoporous SBA-15 particles for in-tube SPME-HILIC-MS/MS to determine levodopa, carbidopa, benserazide, dopamine, and 3-O-methyldopa in plasma samples. Microchemical Journal, v. 157, SEP 2020. (17/02147-0, 17/03726-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GRECCO, Caroline Fernandes. Development of a monolithic phase with amino groups and nanoparticles for the determination of carbidopa, levodopa, benserazide, dopamine and 3-O-methyldopa in plasma samples from patients with Parkinson\'s disease by HILIC-MS / MS in column switching mode. 2019. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.