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Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples


In recent years, modern analytical chemistry has seeked simplification by hyphenation of the techniques, automation (high-throughput performance), and miniaturization of the analytical systems. In this context, column switching liquid chromatography coupled to tandem mass spectrometry (column switching LC-MS/MS) can be highlighted. The development of stationary phases for the first dimension (1D) of this analytical system, such as the restricted access materials (RAM), the molecularly imprinted polymers (MIP), and the polymeric ionic liquids (PIL) have enabled direct biological fluid introduction into chromatographic systems and selective pre-concentration of the analytes. In this project (i) MIP-RAM and PIL phases (1D) were synthesized and evaluated for column switching LC-MS/MS analysis of the endocannabinoids in plasma samples from patients with Parkinson's disease (PD) under therapy with cannabidiol, and (ii) RAM phase (1D) and reverse-phase with core-shell particles (2D) were evaluated the for column switching LC-MS/MS analysis of the drugs (antipsychotics, antidepressants, anticonvulsants, and anxiolytics) in plasma samples from schizophrenic patients. The proposed objectives emerged from two distinct public health problems. The first one concern to evaluate new drugs, such as cannabidiol, to treat PD, since the treatment with dopaminergic drugs, especially levodopa, markedly improves the motor symptoms, but does not halt disease progression. The second problem concerns to schizophrenia, considered as the most serious of the neuropsychiatric upheavals. Among antipsychotics, great part of the schizophrenic patients also makes concomitant use of other classes of drugs, such as antidepressants, anticonvulsants, and anxiolytics, in order to decrease the symptoms associated with this condition. Consequently, the analysis of these drugs in plasma samples from schizophrenic patients is important to adjust the doses, minimize adverse effects and check the patient compliance with treatment. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, ISRAEL D.; HANTAO, LEANDRO W.; QUEIROZ, MARIA EUGENIA C. Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples. Analytica Chimica Acta, v. 1045, p. 108-116, JAN 3 2019. Web of Science Citations: 9.
GRECCO, CAROLINE F.; SOUZA, ISRAEL D.; ACQUARO JUNIOR, VINICIUS R.; QUEIROZ, MARIA E. C. Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 48-59, JAN 2019. Web of Science Citations: 2.
MARCHIONI, CAMILA; DE SOUZA, ISRAEL DONIZETI; ACQUARO JUNIOR, VINICIUS RICARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; TUMAS, VITOR; COSTA QUEIROZ, MARIA EUGENIA. Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases. Analytica Chimica Acta, v. 1044, p. 12-28, DEC 31 2018. Web of Science Citations: 12.
LEMOS PINTO, MONIA AP.; DE SOUZA, ISRAEL D.; QUEIROZ, MARIA EUGENIA C. Determination of drugs in plasma samples by disposable pipette extraction with C18-BSA phase and liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, v. 139, p. 116-124, MAY 30 2017. Web of Science Citations: 10.
MARCHIONI, CAMILA; DONIZETI DE SOUZA, ISRAEL; FERNANDES GRECCO, CAROLINE; ALEXANDRE CRIPPA, JOSE; TUMAS, VITOR; EUGENIA COSTA QUEIROZ, MARIA. A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 409, n. 14, p. 3587-3596, MAY 2017. Web of Science Citations: 8.
ACQUARO JUNIOR, VINICIUS RICARDO; LANCAS, FERNANDO MAURO; COSTA QUEIROZ, MARIA EUGENIA. Evaluation of superficially porous and fully porous columns for analysis of drugs in plasma samples by UHPLC-MS/MS. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1048, p. 1-9, MAR 24 2017. Web of Science Citations: 5.
ACQUARO JUNIOR, VINICIUS RICARDO; DOMINGUES, DIEGO SOARES; COSTA QUEIROZ, MARIA EUGENIA. Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples. BIOANALYSIS, v. 9, n. 6, p. 555-568, MAR 2017. Web of Science Citations: 4.

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