Magnetic stimuli in biotechnological processes

• Grant number: 15/25103-2
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Denise Freitas Siqueira Petri
• Grantee: Denise Freitas Siqueira Petri
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The project aims at understanding the effects of incorporation of magnetic nanoparticles into polymer matrices, in the absence and presence of external magnetic field (CME), on biotechnological processes, such as proliferation and differentiation of neural cells, adsorption and desorption of biomolecules and enzymatic activity of hydrolases. Composites of polysaccharides and magnetic nanoparticles are synthesized in the form of membranes, hydrogels, aerogels and microspheres, and characterized by scanning electron microscopy, thermogravimetric analysis, infrared vibrational spectroscopy and magnetic properties measured. For proliferation and differentiation of neuronal cells on supports made of polysaccharide and magnetic nanoparticles static and pulsed magnetic stimuli will be applied, with the possibility to in situ monitoring cell morphology and Ca2+ ions influx with the help of fluorescence microscope. The comprehension of the adsorption and desorption of levodopa carbidopa and dopamine from composite materials, in the absence and presence of CME, shall be useful to the development of transdermal "patches" for the treatment of neurodegenerative diseases, as well as the differentiation of stem cells into neuronal dopaminergic neurons. Adsorption and desorption of ethinyl estradiol from composite materials will be stimulated by CME. The effect of magnetic stimuli by CME in the enzymatic activity of lipase and cellulase, both hydrolases, will be systematically investigated. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARANTES DE CARVALHO, GABRIEL G.; KONDAVEETI, STALIN; PETRI, DENISE F. S.; FIOROTO, ALEXANDRE M.; ALBUQUERQUE, LUIZA G. R.; OLIVEIRA, PEDRO V.. Evaluation of calcium alginate beads for Ce, La and Nd preconcentration from groundwater prior to ICP OES analysis. Talanta, v. 161, p. 707-712, DEC 1 2016. (15/25103-2, 15/14415-3)

MARTINS, BIANCA F.; DE TOLEDO, PAULO V. O.; PETRI, DENISE F. S.. Hydroxypropyl methylcellulose based aerogels: Synthesis, characterization and application as adsorbents for wastewater pollutants. Carbohydrate Polymers, v. 155, p. 173-181, JAN 2 2017. (15/25103-2)

SHIMADA, ROBSON T.; FONSECA, MATHEUS S.; PETRI, DENISE F. S.. The role of hydroxypropyl methylcellulose structural parameters on the stability of emulsions containing Spirulina biomass. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 529, p. 137-145, SEP 20 2017. (15/25103-2)

KONDAVEETI, STALIN; DE ASSIS BUENO, PEDRO VINICIUS; CARMONA-RIBEIRO, ANA MARIA; ESPOSITO, FERNANDA; LINCOPAN, NILTON; SIERAKOWSKI, MARIA RITA; SIQUEIRA PETRI, DENISE FREITAS. Microbicidal gentamicin-alginate hydrogels. Carbohydrate Polymers, v. 186, p. 159-167, APR 15 2018. (15/25103-2, 15/14415-3)

BUENO, PEDRO V. A.; HILAMATU, KARINA C. P.; CARMONA-RIBEIRO, ANA M.; PETRI, DENISE F. S.. Magnetically triggered release of amoxicillin from xanthan/Fe3O4/albumin patches. International Journal of Biological Macromolecules, v. 115, p. 792-800, AUG 2018. (16/08750-7, 15/25103-2)

CASTRO, POLLYANA S.; BERTOTTI, MAURO; NAVES, ALLINY F.; CATALANI, LUIZ HENRIQUE; CORNEJO, DANIEL R.; BLOISI, GEORGIA D.; PETRI, DENISE F. S.. Hybrid magnetic scaffolds: The role of scaffolds charge on the cell proliferation and Ca2+ ions permeation. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 156, p. 388-396, AUG 1 2017. (15/25103-2)

DE SOUZA, ICARO F. T.; PETRI, DENISE F. S.. beta-Cyclodextrin hydroxypropyl methylcellulose hydrogels for bisphenol A adsorption. JOURNAL OF MOLECULAR LIQUIDS, v. 266, p. 640-648, SEP 15 2018. (15/25103-2)

KONDAVEETI, STALIN; DAMATO, TATIANA C.; CARMONA-RIBEIRO, ANA M.; SIERAKOWSKI, MARIA R.; SIQUEIRA PETRI, DENISE FREITAS. Sustainable hydroxypropyl methylcellulose/xyloglucan/gentamicin films with antimicrobial properties. Carbohydrate Polymers, v. 165, p. 285-293, JUN 1 2017. (15/25103-2, 15/14415-3)

ARANTES DE CARVALHO, GABRIEL G.; KELMER, GISLAYNE A. R.; FARDIM, PEDRO; OLIVEIRA, PEDRO V.; PETRI, DENISE F. S.. Hybrid polysaccharide beads for enhancing adsorption of Cr(VI) ions. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 558, p. 144-153, DEC 5 2018. (15/25103-2, 15/06161-1)

KONDAVEETI, STALIN; PETRI, DENISE F. S.; JEONG, HOON EUI. Efficiency of air-dried and freeze-dried alginate/xanthan beads in batch, recirculating and column adsorption processes. International Journal of Biological Macromolecules, v. 204, p. 11-pg., 2022-04-15. (15/25103-2)

NOVAES, STEPHANIE D.; OLIVEIRA, PEDRO V.; PETRI, DENISE F. S.. Hydroxypropyl Methylcellulose Sponges for the Adsorption of Estrogenic Pollutant. CHEMPLUSCHEM, v. 82, n. 6, p. 904-913, JUN 2017. (15/25103-2)

KONDAVEETI, STALIN; SILVA SEMEANO, ANA TERESA; CORNEJO, DANIEL R.; ULRICH, HENNING; SIQUEIRA PETRI, DENISE FREITAS. Magnetic hydrogels for levodopa release and cell stimulation triggered by external magnetic field. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 167, p. 415-424, JUL 1 2018. (15/25103-2, 15/14415-3)

TOLEDO, PAULO V. O.; PETRI, DENISE F. S.. Hydrophilic, hydrophobic, Janus and multilayer xanthan based cryogels. International Journal of Biological Macromolecules, v. 123, p. 1180-1188, FEB 15 2019. (15/25103-2)

TOLEDO, PAULO V. O.; MARTINS, BIANCA F.; PIRICH, CLEVERTON L.; SIERAKOWSKI, MARIA RITA; TEIXEIRA NETO, ERICO; PETRI, DENISE F. S.. Cellulose Based Cryogels as Adsorbents for Organic Pollutants. MACROMOLECULAR SYMPOSIA, v. 383, n. 1, p. 12-pg., 2019-02-01. (15/25103-2)