Magnetic hydrogels for levodopa release and cell stimulation triggered by external magnetic field

Author(s)

Kondaveeti, Stalin ^[1] ; Silva Semeano, Ana Teresa ^[2] ; Cornejo, Daniel R. ^[3] ; Ulrich, Henning ^[2] ; Siqueira Petri, Denise Freitas ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 167, p. 415-424, JUL 1 2018.
• Web of Science Citations: 9

Abstract

Magnetic responsive hydrogels composed of alginate (Alg) and xanthan gum (XG), crosslinked with Ca2+ ions, were modified by in situ magnetic nanoparticles (MNP) formation. In comparison to magnetic Alg hydrogels, magnetic Alg-XG hydrogels presented superior mechanical and swelling properties, due to the high charge density and molecular weight of XG. The loading efficiency of levodopa (LD), an important antiparkinson drug, in the Alg-XG/MNP hydrogels was the highest (64%), followed by Alg/MNP (56%), Alg-XG (53%) and Alg (28%). A static external magnetic field (EMF) of 0.4T stimulated the release of LD from Alg-XG/MNP hydrogels achieving 64 +/- 6% of the initial loading after 30 h. The viability, proliferation and expression of dopaminergic markers of human neuroblastoma SH-SY5Y cell on the LD loaded magnetic hydrogels were successful, particularly under EMF, which stimulated the release of LD. Overall, the results of this study provided the rational design of magnetic hydrogels for the delivery of drugs, which combined with external magnetic stimulus, might improve cell proliferation and specific differentiation. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/25103-2 - Magnetic stimuli in biotechnological processes
• Grantee: Denise Freitas Siqueira Petri
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/14415-3 - Preparation of multicomponent polymeric magnetic materials for biotechnological applications
• Grantee: Stalin Kondaveeti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral