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Surface chemistry effects on the formation of protein corona over nano-sized silver colloids and its implications on cytotoxicity and biomedical applications

Grant number: 17/24603-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2018
Status:Discontinued
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Fernando Carlos Giacomelli
Grantee:Carin Cristina da Silva Batista
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Associated scholarship(s):19/03223-7 - Facing the polydispersity of POLYMER-STABILIZED silver NANOCOLLOIDS by using microfluidics with further investigations on the thermodynamics of protein adsorption, BE.EP.DR

Abstract

These investigations will be devoted to the manufacturing of nano-sized silver colloids (AgNPs) stabilized by different amino-functionalized polymers and the influence of the capping agents and other nanoparticle´s features (size, surface charge, etc.) on the adsorption of plasma proteins. Initially, AgNPs will be produced in the presence of different polyamines acting simultaneously as reductant and stabilizer agent. The assemblies will be further characterized by using scattering techniques (Dynamic Light Scattering - DLS, Static Light Scattering - SLS, Electrophoretic Light Scattering - ELS) besides Transmission Electron Microscopy (TEM) and UV-vis spectroscopy. Subsequently, the adsorption of proteins (HSA, IgG, fibrinogen, APoA1 and lysozyme) onto the surface of the metallic colloids will be investigated taking into account binding dynamics, binding ratio, thermodynamic aspects of binding and possible protein conformational changes promoted by the adsorption phenomena. In the step further, the produced AgNPs will be incubated in the whole plasma environment and by using centrifugation, polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS) it will be identified and quantified the adsorbed macromolecules onto the surface of the colloids. Finally, the cytotoxicity and antimicrobial effects of the protein-nanoparticle complexes will be evaluated and compared to the naked counterparts in order to shed light in the impact of the protein corona on such properties. (AU)