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Facing the polydispersity of polymer-stabilized silver nanocolloids by using microfluidics with further investigations on the thermodynamics of protein adsorption

Grant number: 19/03223-7
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): August 01, 2019
Effective date (End): July 31, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Fernando Carlos Giacomelli
Grantee:Carin Cristina da Silva Batista
Supervisor: Petr Stepanek
Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Research place: Czech Academy of Sciences (CAS), Czech Republic  
Associated to the scholarship:17/24603-7 - Surface chemistry effects on the formation of protein corona over nano-sized silver colloids and its implications on cytotoxicity and biomedical applications, BP.DR


Considering biomedical research, the potential application of nanoparticles recently created a new and emerging area of investigations named nanomedicine where nanoparticles are evaluated to be used in the delivering of active agents and diagnostics. In the bloodstream, the nanoassemblies inevitably become coated by a protein layer and this greatly impact many biological processes including cytotoxicity, cellular uptake, circulation lifetime, signaling, therapeutic effects, intracellular transport and biodistribution. Accordingly, this proposal aims to a better control on the structural features of polymer-stabilized silver colloids by conducting the synthesis using microfluidics with further investigations regarding thermodynamics of protein adsorption. During the experimental activities within the framework of the project 2017/24603-7, we faced considerable difficulties to produce polymer-stabilized AgNPs with low polydispersity indexes and the main motivation regards to the use of microfluidic approaches available at the Institute of Macromolecular Chemistry (Prague - Czech Republic) to more precisely control of the structural properties. The AgNPs will be further characterized in detail by using a variety of scattering techniques, spectroscopy and imaging. Subsequently, we will investigate the thermodynamics of protein adsorption using the resources of isothermal titration calorimetry (ITC). This facility is still unavailable at our University in Brazil and therefore we considered appropriate to include such experimental activities in the proposed work flow and timing. The interaction and adsorption of distinct proteins (HSA, IgG and lysozyme) will be investigated taking into account binding dynamics, binding affinity and binding ratio towards a better understanding of the behavior of polymer-stabilized metallic colloids in biological environments. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, CARIN C. S.; JAGER, ALESSANDRO; ALBUQUERQUE, BRUNNO L.; PAVLOVA, EWA; STEPANEK, PETR; GIACOMELLI, FERNANDO C.. Microfluidic-assisted synthesis of uniform polymer-stabilized silver colloids. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 618, . (19/03223-7, 17/24603-7)
BATISTA, CARIN C. S.; PANICO, KARINE; TROUSIL, JIRI; JANOUSKOVA, OLGA; CASTRO, CARLOS EDUARDO DE; STEPANEK, PETR; GIACOMELLI, FERNANDO C.. Protein coronas coating polymer-stabilized silver nanocolloids attenuate cytotoxicity with minor effects on antimicrobial performance. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 218, p. 11-pg., . (19/06634-8, 21/12071-6, 17/24603-7, 19/03223-7)
BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; JAGER, ALESSANDRO; STEPANEK, PETR; GIACOMELLI, FERNANDO C.. Probing protein adsorption onto polymer-stabilized silver nanocolloids towards a better understanding on the evolution and consequences of biomolecular coronas. Materials Science & Engineering C-Materials for Biological Applications, v. 111, . (17/24603-7, 19/03223-7, 17/00459-4)

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