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Biophysicochemical interactions at the nanobiointerface: the effect of surface features and protein corona on the cellular uptake of polymeric assemblies

Abstract

The investigations will be devoted to cellular uptake of nanoscale structures and the influence of their surface features and the presence of the protein corona on the phenomenon. Initially, a library of coumarin-6 loaded polymeric nanoparticles will be manufactured by using the pH-responsive and biocompatible block copolymers PEOm-b-PDPAn, PMPCm-b-PDPAn and PHPMAm-b-PDPAn. They will be deeply characterized by using essentially scattering techniques (dynamic light scattering - DLS, static light scattering - SLS and electrophoretic light scattering - ELS) besides transmission electron microscopy (TEM). Subsequently, it will be evaluated the cellular uptake of the produced entities and the relation with their structural features, particularly the nature of the hydrophilic stabilizing shell (PEO, PMPC and PHPMA). These investigations will be performed using the resources of flow cytometry (FACS) and laser scanning confocal microscopy (LSCM). Afterwards, the focus will be moved to the protein corona formation. The produced nanoparticles will be incubated in plasma environment and by using centrifugation, polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS) it will be identified and quantified the adsorbed macromolecules onto the distinct entities. The cellular uptake of the protein-nanoparticle complexes will be further investigated in order to shed some light on its effect. These investigations will be complemented by using Fluorescence Lifetime Correlation Spectroscopy (FLCS) in order to evaluate the adsorption of the most abundant plasma protein (Human Serum Albumin - HSA) with the produced assemblies. The fundamental goal of the proposal is to understand essentially how the surface chemistry of nanoassemblies and the protein corona influence the uptaken behavior of the entities. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, FERNANDO A.; ALBUQUERQUE, LINDOMAR J. C.; RISKE, KARIN A.; JAGER, ELIEZER; GIACOMELLI, FERNANDO C.. Outstanding protein-repellent feature of soft nanoparticles based on poly(N-(2-hydroxypropyl) methacrylamide) outer shells. Journal of Colloid and Interface Science, v. 574, p. 260-271, . (17/19079-7, 18/21272-2, 17/00459-4)
BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; JAGER, ALESSANDRO; STEPANEK, PETR; GIACOMELLI, FERNANDO C.. Probing protein adsorption onto polymer-stabilized silver nanocolloids towards a better understanding on the evolution and consequences of biomolecular coronas. Materials Science & Engineering C-Materials for Biological Applications, v. 111, . (17/24603-7, 19/03223-7, 17/00459-4)
RIBEIRO, CAROLINE A. S.; ALBUQUERQUE, LINDOMAR J. C.; DE CASTRO, CARLOS E.; BATISTA, BRUNO L.; DE SOUZA, ANDRE L. M.; ALBUQUERQUE, BRUNNO L.; ZILSE, MORGANA S.; BELLETTINI, ISMAEL C.; GIACOMELLI, FERNANDO C.. One-pot synthesis of sugar-decorated gold nanoparticles with reduced cytotoxicity and enhanced cellular uptake. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 580, . (17/00459-4)
ALBUQUERQUE, LINDOMAR J. C.; SINCARI, VLADIMIR; JAGER, ALESSANDRO; KONEFAL, RAFAL; PANEK, JIRI; CERNOCH, PETER; PAVLOVA, EWA; STEPANEK, PETR; GIACOMELLI, FERNANDO C.; JAGER, ELIEZER. Microfluidic-Assisted Engineering of Quasi-Monodisperse pH-Responsive Polymersomes toward Advanced Platforms for the Intracellular Delivery of Hydrophilic Therapeutics. Langmuir, v. 35, n. 25, p. 8363-8372, . (17/11261-0, 17/00459-4)
DOS SANTOS, MARINALVA CARDOSO; KROETZ, THAIS; DORA, CRISTIANA LIMA; GIACOMELLI, FERNANDO CARLOS; ALLIEVI FRIZON, TIAGO ELIAS; PICH, CLAUS TROGER; PINTO, LUCIANO DA SILVA; SOARES, AMANDA SIGAL; RODEMBUSCH, FABIANO SEVERO; DE LIMA, VANIA RODRIGUES; et al. Elucidating Bauhinia variegata lectin/phosphatidylcholine interactions in lectin-containing liposomes. Journal of Colloid and Interface Science, v. 519, p. 232-241, . (17/00459-4)
BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; DE ARAUJO, IRIS; ALBUQUERQUE, BRUNNO L.; DA SILVA, FERNANDA D.; GIACOMELLI, FERNANDO C.. Antimicrobial activity of nano-sized silver colloids stabilized by nitrogen-containing polymers: the key influence of the polymer capping. RSC ADVANCES, v. 8, n. 20, p. 10873-10882, . (17/00459-4, 16/23844-8)
DU, HAIQIN; DE OLIVEIRA, FERNANDO A.; ALBUQUERQUE, LINDOMAR J. C.; TRESSET, GUILLAUME; PAVLOVA, EWA; HUIN, CECILE; GUEGAN, PHILIPPE; GIACOMELLI, FERNANDO C.. Polyglycidol-Stabilized Nanoparticles as a Promising Alternative to Nanoparticle PEGylation: Polymer Synthesis and Protein Fouling Considerations. Langmuir, v. 36, n. 5, p. 1266-1278, . (18/11038-2, 17/00459-4)
DE CASTRO, CARLOS E.; RIBEIRO, CAROLINE A. S.; DA SILVA, MARIA C. C.; DAL-BO, ALEXANDRE GONCALVES; GIACOMELLI, FERNANDO C.. Sweetness Reduces Cytotoxicity and Enables Faster Cellular Uptake of Sub-30 nm Amphiphilic Nanoparticles. Langmuir, v. 35, n. 24, p. 8060-8067, . (15/24686-4, 17/00459-4)
JAGER, ELIEZER; SINCARI, VLADIMIR; ALBUQUERQUE, LINDOMAR J. C.; JAGER, ALESSANDRO; HUMAJOVA, JANA; KUCKA, JAN; PANKRAC, JAN; PARAL, PETR; HEIZER, TOMAS; JANOUSKOVA, OLGA; et al. Reactive Oxygen Species (ROS)-Responsive Polymersomes with Site-Specific Chemotherapeutic Delivery into Tumors via Spacer Design Chemistry. Biomacromolecules, v. 21, n. 4, p. 1437-1449, . (17/00459-4, 17/11261-0)
DE CASTRO, CARLOS E.; RIBEIRO, CAROLINE A. S.; ALAVARSE, ALEX C.; ALBUQUERQUE, LINDOMAR J. C.; DA SILVA, MARIA C. C.; JAEGER, ELIEZER; SURMAN, FRANTISEK; SCHMIDT, VANESSA; GIACOMELLI, CRISTIANO; GIACOMELLI, FERNANDO C.. Nanoparticle-Cell Interactions: Surface Chemistry Effects on the Cellular Uptake of Biocompatible Block Copolymer Assemblies. Langmuir, v. 34, n. 5, p. 2180-2188, . (15/24686-4, 17/00459-4)
ALBUQUERQUE, LINDOMAR J. C.; ALAVARSE, ALEX C.; CARLAN DA SILVA, MARIA C.; ZILSE, MORGANA S.; BARTH, MAITE T.; BELLETTINI, ISMAEL C.; GIACOMELLI, FERNANDO C.. Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression. Macromolecular Bioscience, v. 18, n. 2, . (16/23844-8, 17/00459-4)

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