Genome and transcriptome analyses of Leishmania spp.: opening Pandora's box

Autor(es)

Cruz, Angela Kaysel ^[1] ; Freitas-Castro, Felipe ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Current Opinion in Microbiology; v. 52, p. 64-69, DEC 2019.
• Citações Web of Science: 0

Resumo

In the last 30 years, significant advances in genetic manipulation tools along with complete genome and transcriptome sequencing have advanced our understanding of the biology of Leishmania parasites and their interplay with the sand fly and mammalian hosts. High-throughput sequencing in association with CRISPR/Cas9 have prepared the ground for significant advances. Given the richness of the progress made over the last decade, in this article, we focused on the most recent contributions of genome-wide and transcriptome analyses of Leishmania spp., which permit the comparison of life cycle stages, the evaluation of different strains and species in their natural niches and in the field and the simultaneously comparison of the gene expression profiles of parasites and hosts. (AU)

• Processo FAPESP: 13/50219-9 - Investigação sobre fatores e mecanismos do controle de expressão gênica em Leishmania: do papel de modificações pós-traducionais, RNAs não codificadores, cis-elementos e amplificação gênica
• Beneficiário: Angela Kaysel Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Temático