Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane

Autor(es)

Dantas, Luiza L. B. ^{[1, 2]} ; Calixto, Cristiane P. G. ^[3] ; Dourado, Maira M. ^[1] ; Carneiro, Monalisa S. ^[4] ; Brown, John W. S. ^{[3, 5]} ; Hotta, Carlos T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[2] Max Planck Inst Mol Plant Physiol, Potsdam - Germany

^[3] Univ Dundee, James Hutton Inst, Sch Life Sci, Div Plant Sci, Dundee - Scotland

^[4] Univ Fed Sao Carlos, Dept Biotecnol Prod Vegetal & Anim, Ctr Ciencias Agr, Araras - Brazil

^[5] James Hutton Inst, Cell & Mol Sci, Dundee - Scotland

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 10, DEC 23 2019.
• Citações Web of Science: 0

Resumo

Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice, and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to test if the daily dynamics of AS forms of circadian clock genes are regulated by environmental factors, such as temperature, in the field. A systematic search for AS in five sugarcane clock genes, ScLHY, ScPRR37, ScPRR73, ScPRR95, and ScTOC1 using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are correlated with temperature. (AU)

• Processo FAPESP: 15/10220-3 - Revelando as relações entre relógio biológico, splicing alternativo e metabolismo da cana-de-açúcar
• Beneficiário: Luíza Lane de Barros Dantas
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
• Beneficiário: Carlos Takeshi Hotta
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 11/00818-8 - Desenvolvimento de modelos biológicos alternativos para o estudo de redes de regulação em cana-de-açúcar
• Beneficiário: Carlos Takeshi Hotta
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 11/08897-4 - Caracterização do relógio biológico e seu impacto no metabolismo da cana-de-açúcar
• Beneficiário: Luíza Lane de Barros Dantas
• Modalidade de apoio: Bolsas no Brasil - Doutorado