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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane

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Author(s):
Dantas, Luiza L. B. [1, 2] ; Calixto, Cristiane P. G. [3] ; Dourado, Maira M. [1] ; Carneiro, Monalisa S. [4] ; Brown, John W. S. [3, 5] ; Hotta, Carlos T. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[2] Max Planck Inst Mol Plant Physiol, Potsdam - Germany
[3] Univ Dundee, James Hutton Inst, Sch Life Sci, Div Plant Sci, Dundee - Scotland
[4] Univ Fed Sao Carlos, Dept Biotecnol Prod Vegetal & Anim, Ctr Ciencias Agr, Araras - Brazil
[5] James Hutton Inst, Cell & Mol Sci, Dundee - Scotland
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 10, DEC 23 2019.
Web of Science Citations: 0
Abstract

Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice, and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to test if the daily dynamics of AS forms of circadian clock genes are regulated by environmental factors, such as temperature, in the field. A systematic search for AS in five sugarcane clock genes, ScLHY, ScPRR37, ScPRR73, ScPRR95, and ScTOC1 using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are correlated with temperature. (AU)

FAPESP's process: 15/10220-3 - Unveiling the relationships among circadian clock, alternative splicing and sugarcane metabolism
Grantee:Luíza Lane de Barros Dantas
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 15/06260-0 - Organ- and tissue- specific circadian clocks in C4 grasses
Grantee:Carlos Takeshi Hotta
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 11/00818-8 - Development of alternative biological models for the study of sugarcane regulatory networks
Grantee:Carlos Takeshi Hotta
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 11/08897-4 - Characterization of the sugarcane circadian clock and its impact on the metabolism
Grantee:Luíza Lane de Barros Dantas
Support type: Scholarships in Brazil - Doctorate