Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis

Autor(es):Mostrar menos -

Costa, Victor ^[1] ; El-Achkar, Vivian Narana ^[1] ; de Barros, Patricia Pimentel ^[1] ; Leon, Jorge Esquiche ^[2] ; Ribeiro-Silva, Alfredo ^[3] ; Carlos, Roman ^[4] ; Nagata Pignatari, Shirley Shizue ^[5] ; Ferreira, Silvaneide ^[6] ; Mello, Barbara Pereira ^[7] ; Sichero, Laura ^[6] ; Villa, Luisa Lina ^{[7, 6]} ; Kaminagakura, Estela ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, UNESP, Sao Jose Dos Campos, SP - Brazil

^[2] Ribeirao Preto Sch Dent, Dept Stomatol Collect Hlth & Legal Dent, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Forens Med, Ribeirao Preto - Brazil

^[4] Ctr Clin Cabeza & Cuello, Guatemala City - Guatemala

^[5] Univ Fed Sao Paulo, Dept Otolaryngol & Head & Neck Surg, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Ctr Translat Invest Oncol, Inst Canc Estado Sao Paulo, Fac Med, Hosp Clin, Sao Paulo, SP - Brazil

^[7] Univ Sao Paulo, Fac Med, Dept Radiol & Oncol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LARYNGOSCOPE; v. 130, n. 11 DEC 2019.
• Citações Web of Science: 2

Resumo

Objective The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale >= 20) (P = 0.04). Conclusion The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence 4 Laryngoscope, 2019 (AU)

• Processo FAPESP: 16/24019-0 - Investigação da presença de epstein-barr vírus e sua possível contribuição na agressividade e malignização da papilomatose laríngea
• Beneficiário: Estela Kaminagakura Tango
• Modalidade de apoio: Auxílio à Pesquisa - Regular