Impact of sublethal doses of thiamethoxam and Nosema ceranae inoculation on the hepato-nephrocitic system in young Africanized Apis mellifera

Autor(es)

Balsamo, Paulo Jose ^{[1, 2]} ; da Costa Domingues, Caio Eduardo ^[1] ; da Silva-Zacarin, Elaine Cristina Mathias ^[1] ; Gregorc, Ales ^{[3, 4]} ; Irazusta, Silvia Pierre ^[2] ; Salla, Raquel Fernanda ^[1] ; Costa, Monica Jones ^[1] ; Abdalla, Fabio Camargo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, PPGBMA, Campus Sorocaba, Sao Paulo, SP - Brazil

^[2] Fac Technol Sorocaba, Coordinat Biomed Syst, Sao Paulo, SP - Brazil

^[3] Agr Inst Slovenia, Ljubljana - Slovenia

^[4] Mississippi State Univ, Ctr Costal Hort Res, Poplarville, MS - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APICULTURAL RESEARCH; v. 59, n. 4 NOV 2019.
• Citações Web of Science: 2

Resumo

We analyzed the morphological changes on the hepato-nephrocitic system (HNS) of Africanized honey bees exposed to thiamethoxam (TMX) and to Nosema ceranae (NOS) in isolation and co-exposure. We also analyzed the relative amount of hemocytes in TMX and/or Nosema-exposed bees. Newly emerged bees were exposed individually per os to TMX, at doses of 0.0856 ng/bee or 0.00856 ng/bee, or to TMX doses and 60,000 spores of N. ceranae per bee. Eight days after the single exposure, the results showed that TMX was extremely deleterious to the HNS at 0.00856 ng/bee, dramatically inducing morphological changes in HNS cells (trophocytes and oenocytes) and increasing hemocyte number. However, the treatment to TMX at 0.00856 ng/bee with additional NOS inoculation provoked the highest critical damage observed on HNS, collapsing the trophocytes, reducing the surface area of oenocytes, and inducing cell death in pericardial cells. Therefore, a sublethal dose of TMX, applied alone or simultaneously with NOS inoculation, triggered the disruption of the HNS and induced progressive damage on worker bees. The results demonstrated an interaction between biotic and abiotic agents in Africanized bees at individual level, whose impact at the colonial level remains to be assessed under field conditions. (AU)

• Processo FAPESP: 14/04697-9 - Efeitos da interação entre o inseticida tiametoxam e o fungicida picoxistrobina em larvas e adultos de Apis mellifera africanizada
• Beneficiário: Caio Eduardo da Costa Domingues
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/06056-9 - Ação do mercúrio no sistema hepatonefrocítico de Bombus atratus Franklin (Hymenoptera: Bombini)
• Beneficiário: Fábio Camargo Abdalla
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17840-6 - Ação do cádmio e do Roundup® original em órgãos internos de Bombus morio e Bombus atratus (Hymenoptera: Bombini)
• Beneficiário: Fábio Camargo Abdalla
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/09419-4 - Interação entre pesticidas e infecção por Nosema em Apis mellifera africanizada: efeitos biológicos e detecção de biomarcadores celulares
• Beneficiário: Elaine Cristina Mathias da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular