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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of sublethal doses of thiamethoxam and Nosema ceranae inoculation on the hepato-nephrocitic system in young Africanized Apis mellifera

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Author(s):
Balsamo, Paulo Jose [1, 2] ; da Costa Domingues, Caio Eduardo [1] ; da Silva-Zacarin, Elaine Cristina Mathias [1] ; Gregorc, Ales [3, 4] ; Irazusta, Silvia Pierre [2] ; Salla, Raquel Fernanda [1] ; Costa, Monica Jones [1] ; Abdalla, Fabio Camargo [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, PPGBMA, Campus Sorocaba, Sao Paulo, SP - Brazil
[2] Fac Technol Sorocaba, Coordinat Biomed Syst, Sao Paulo, SP - Brazil
[3] Agr Inst Slovenia, Ljubljana - Slovenia
[4] Mississippi State Univ, Ctr Costal Hort Res, Poplarville, MS - USA
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF APICULTURAL RESEARCH; v. 59, n. 4 NOV 2019.
Web of Science Citations: 2
Abstract

We analyzed the morphological changes on the hepato-nephrocitic system (HNS) of Africanized honey bees exposed to thiamethoxam (TMX) and to Nosema ceranae (NOS) in isolation and co-exposure. We also analyzed the relative amount of hemocytes in TMX and/or Nosema-exposed bees. Newly emerged bees were exposed individually per os to TMX, at doses of 0.0856 ng/bee or 0.00856 ng/bee, or to TMX doses and 60,000 spores of N. ceranae per bee. Eight days after the single exposure, the results showed that TMX was extremely deleterious to the HNS at 0.00856 ng/bee, dramatically inducing morphological changes in HNS cells (trophocytes and oenocytes) and increasing hemocyte number. However, the treatment to TMX at 0.00856 ng/bee with additional NOS inoculation provoked the highest critical damage observed on HNS, collapsing the trophocytes, reducing the surface area of oenocytes, and inducing cell death in pericardial cells. Therefore, a sublethal dose of TMX, applied alone or simultaneously with NOS inoculation, triggered the disruption of the HNS and induced progressive damage on worker bees. The results demonstrated an interaction between biotic and abiotic agents in Africanized bees at individual level, whose impact at the colonial level remains to be assessed under field conditions. (AU)

FAPESP's process: 13/09419-4 - Interaction between pesticides and Nosema infection in Africanized Apis mellifera: biological effects and detection of cellular biomarkers
Grantee:Elaine Cristina Mathias da Silva Zacarin
Support type: Regular Research Grants
FAPESP's process: 14/04697-9 - Effects of the interaction between the thiametoxam insecticide and picoxystrobin fungicide on larvae and adult of africanized Apis mellifera
Grantee:Caio Eduardo da Costa Domingues
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/06056-9 - Effect of mercury on the hepato-nephrocitic system of Bombus atratus Franklin (Hymenoptera: Bombini)
Grantee:Fábio Camargo Abdalla
Support type: Regular Research Grants
FAPESP's process: 11/17840-6 - Effect of cadmium and Roundup® on internal organs of Bombus morio and Bombus atratus (Hymenoptera: Bombini)
Grantee:Fábio Camargo Abdalla
Support type: Regular Research Grants